Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection

E.A. Bruijn, de; U.R. Tjaden; A.T. Oosterom, van; P. Leeflang; P.A. Leclercq

doi:10.1016/S0021-9673%2801%2993663-5

Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection

E.A. Bruijn, de, U.R. Tjaden, A.T. Oosterom, van, P. Leeflang, P.A. Leclercq

Chemical Engineering and Chemistry

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A rapid and sensitive method for the determination of cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolises in one analysis has been developed. Surface-coated open-tubular OV-275 columns were combined with electron-capture detection and nitrogen phosphorus selective detection. The influence of the column diameter on the separation is shown. Extraction with 2-propanol-diethyl ether (22:77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay was applied to some pharmacokinetic experiments with chemotherapeutically treated patients and with a WAG/Rij rat.

Original language	English
Pages (from-to)	603-608
Journal	Journal of Chromatography
Volume	279
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673%2801%2993663-5
Publication status	Published - 1983

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Bruijn, de, E. A., Tjaden, U. R., Oosterom, van, A. T., Leeflang, P., & Leclercq, P. A. (1983). Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection. Journal of Chromatography, 279(1), 603-608. https://doi.org/10.1016/S0021-9673%2801%2993663-5

@article{d58a083c526a44938b09a088cd716442,

title = "Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection",

abstract = "A rapid and sensitive method for the determination of cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolises in one analysis has been developed. Surface-coated open-tubular OV-275 columns were combined with electron-capture detection and nitrogen phosphorus selective detection. The influence of the column diameter on the separation is shown. Extraction with 2-propanol-diethyl ether (22:77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay was applied to some pharmacokinetic experiments with chemotherapeutically treated patients and with a WAG/Rij rat.",

author = "{Bruijn, de}, E.A. and U.R. Tjaden and {Oosterom, van}, A.T. and P. Leeflang and P.A. Leclercq",

year = "1983",

doi = "10.1016/S0021-9673%2801%2993663-5",

language = "English",

volume = "279",

pages = "603--608",

journal = "Journal of Chromatography",

issn = "0021-9673",

publisher = "Elsevier",

number = "1",

}

Bruijn, de, EA, Tjaden, UR, Oosterom, van, AT, Leeflang, P & Leclercq, PA 1983, 'Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection', Journal of Chromatography, vol. 279, no. 1, pp. 603-608. https://doi.org/10.1016/S0021-9673%2801%2993663-5

Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection. / Bruijn, de, E.A.; Tjaden, U.R.; Oosterom, van, A.T. et al.
In: Journal of Chromatography, Vol. 279, No. 1, 1983, p. 603-608.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection

AU - Bruijn, de, E.A.

AU - Tjaden, U.R.

AU - Oosterom, van, A.T.

AU - Leeflang, P.

AU - Leclercq, P.A.

PY - 1983

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AB - A rapid and sensitive method for the determination of cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolises in one analysis has been developed. Surface-coated open-tubular OV-275 columns were combined with electron-capture detection and nitrogen phosphorus selective detection. The influence of the column diameter on the separation is shown. Extraction with 2-propanol-diethyl ether (22:77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay was applied to some pharmacokinetic experiments with chemotherapeutically treated patients and with a WAG/Rij rat.

U2 - 10.1016/S0021-9673%2801%2993663-5

DO - 10.1016/S0021-9673%2801%2993663-5

M3 - Article

SN - 0021-9673

VL - 279

SP - 603

EP - 608

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1

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Bruijn, de EA, Tjaden UR, Oosterom, van AT, Leeflang P, Leclercq PA. Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection. Journal of Chromatography. 1983;279(1):603-608. doi: 10.1016/S0021-9673%2801%2993663-5