Determination of the underivatized antineoplastic drugs cyclophosphamide and 5-fluorouracil and some of their metabolites by capillary gas chromatography combined with electron-capture and nitrogen-phosphorus selective detection

E.A. Bruijn, de, U.R. Tjaden, A.T. Oosterom, van, P. Leeflang, P.A. Leclercq

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Abstract

A rapid and sensitive method for the determination of cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolises in one analysis has been developed. Surface-coated open-tubular OV-275 columns were combined with electron-capture detection and nitrogen phosphorus selective detection. The influence of the column diameter on the separation is shown. Extraction with 2-propanol-diethyl ether (22:77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay was applied to some pharmacokinetic experiments with chemotherapeutically treated patients and with a WAG/Rij rat.
Original languageEnglish
Pages (from-to)603-608
JournalJournal of Chromatography, A
Volume279
Issue number1
DOIs
Publication statusPublished - 1983

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