Determination of the underivatized antineoplastic drugs CP, 5-FU and somE of their metabolites by capillary gas chromatography combined with electron capture and nitrogen-phosphorous selective detection

E.A. Bruijn, de, U.R. Tjaden, A.T. Oosterom, van, P. Leeflang, P.A. Leclercq

Research output: Chapter in Book/Report/Conference proceedingConference contributionProfessional

Abstract

A rapid and sensitive method to determine cyclophosphamide (CP) and 5-fluorouracil (5-FU) and some of their metabolites in one run has been developed. SCOT OV 275 columns were combined with Electron Capture Detection (ECD) and Nitrogen Phosphorus Selective Detection (NPSD). The influence of the column diameter on the separation is shown. The extraction with i-propanol/ether (22/77) allows the isolation of CP, 5-FU and their analogues in one extraction step. The assay is applied to some pharmacokinetic experiments concerning chemotherapeutically treated patients and one WAG/Rij rat.
Original languageEnglish
Title of host publicationProceedings of the 5th International symposium on capillary gas chromatography, Riva del Garda, Italy
EditorsJ.A. Rijks
Place of PublicationAmsterdam
PublisherElsevier
Pages693-701
Publication statusPublished - 1983
Event5th International Symposium on Capillary Chromatography, April 26-28, 1983, Riva del Garda, Italy - Riva del Garda, Italy
Duration: 26 Apr 198328 Apr 1983

Conference

Conference5th International Symposium on Capillary Chromatography, April 26-28, 1983, Riva del Garda, Italy
CountryItaly
CityRiva del Garda
Period26/04/8328/04/83

Fingerprint

Dive into the research topics of 'Determination of the underivatized antineoplastic drugs CP, 5-FU and somE of their metabolites by capillary gas chromatography combined with electron capture and nitrogen-phosphorous selective detection'. Together they form a unique fingerprint.

Cite this