Determination of particle-particle distances in an optomagnetic cluster assay: a rotating molecular ruler

R.W.L. Vliembergen, van, L.J. IJzendoorn, van, M.W.J. Prins

Research output: Contribution to conferencePosterAcademic

Abstract

We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations. [1,2] In this paper we report a method to quantify the particle-particle distance with nanometer resolution. At higher scattering angles, the signal contains higher frequency components. Simulations of the light scattering by rotating clusters show that the high-frequency Fourier components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance. We will report the simulation results and experimental data of model cluster assays with controlled particle-particle distances. 1: Ranzoni et al, Nano Letters 2011 11 (5), 2017- 2022 2: Ranzoni et al, ACS Nano 2012 6 (4), 3134-3141
Original languageEnglish
Publication statusPublished - 2014
Eventconference; NanoCity, 27-28 October 2014, Utrecht, NL; 2014-10-27; 2014-10-28 -
Duration: 27 Oct 201428 Oct 2014

Conference

Conferenceconference; NanoCity, 27-28 October 2014, Utrecht, NL; 2014-10-27; 2014-10-28
Period27/10/1428/10/14
OtherNanoCity, 27-28 October 2014, Utrecht, NL

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    Vliembergen, van, R. W. L., IJzendoorn, van, L. J., & Prins, M. W. J. (2014). Determination of particle-particle distances in an optomagnetic cluster assay: a rotating molecular ruler. Poster session presented at conference; NanoCity, 27-28 October 2014, Utrecht, NL; 2014-10-27; 2014-10-28, .