We investigate a fast and sensitive optomagnetic bionanotechnology for biomarker detection. Antibody-coated superparamagnetic particles capture biomarker molecules and form clusters with a biomarker molecule sandwiched between two particles. These particle clusters can be analyzed using a rotating magnetic field which induces an oscillating light scattering cross-section, resolving sub-picomolar biomarker concentrations. [1,2] In this paper we report a method to quantify the particle-particle distance with nanometer resolution. At higher scattering angles, the signal contains higher frequency components. Simulations of the light scattering by rotating clusters show that the high-frequency Fourier components hold detailed information about the geometry of the particle clusters, including a strong dependence on the inter-particle distance. We will report the simulation results and experimental data of model cluster assays with controlled particle-particle distances. 1: Ranzoni et al, Nano Letters 2011 11 (5), 2017- 2022 2: Ranzoni et al, ACS Nano 2012 6 (4), 3134-3141
|Publication status||Published - 2014|
|Event||conference; NanoCity, 27-28 October 2014, Utrecht, NL; 2014-10-27; 2014-10-28 - |
Duration: 27 Oct 2014 → 28 Oct 2014
|Conference||conference; NanoCity, 27-28 October 2014, Utrecht, NL; 2014-10-27; 2014-10-28|
|Period||27/10/14 → 28/10/14|
|Other||NanoCity, 27-28 October 2014, Utrecht, NL|
Vliembergen, van, R. W. L., IJzendoorn, van, L. J., & Prins, M. W. J. (2014). Determination of particle-particle distances in an optomagnetic cluster assay: a rotating molecular ruler. Poster session presented at conference; NanoCity, 27-28 October 2014, Utrecht, NL; 2014-10-27; 2014-10-28, .