Determination of mouse skeletal muscle architecture using three dimensional diffusion tensor imaging

A.M. Heemskerk, G.J. Strijkers, A. Vilanova, M.R. Drost, K. Nicolaij

Research output: Contribution to journalArticleAcademicpeer-review

121 Citations (Scopus)

Abstract

Muscle architecture is the main determinant of the mechanical behavior of skeletal muscles. This study explored the feasibility of diffusion tensor imaging (DTI) and fiber tracking to noninvasively determine the in vivo three-dimensional (3D) architecture of skeletal muscle in mouse hind leg. In six mice, the hindlimb was imaged with a diffusion-weighted (DW) 3D fast spin-echo (FSE) sequence followed by the acquisition of an exercise-induced, T2-enhanced data set. The data showed the expected fiber organization, from which the physiological cross-sectional area (PCSA), fiber length, and pennation angle for the tibialis anterior (TA) were obtained. The values of these parameters ranged from 5.4-9.1 mm2, 5.8-7.8 mm, and 21-24°, respectively, which is in agreement with values obtained previously with the use of invasive methods. This study shows that 3D DT acquisition and fiber tracking is feasible for the skeletal muscle of mice, and thus enables the quantitative determination of muscle architecture.
Original languageEnglish
Pages (from-to)1333-1340
JournalMagnetic Resonance in Medicine
Volume53
Issue number6
DOIs
Publication statusPublished - 2005

Fingerprint Dive into the research topics of 'Determination of mouse skeletal muscle architecture using three dimensional diffusion tensor imaging'. Together they form a unique fingerprint.

  • Cite this