Determination of diffusion coefficients and separation numbers in micellar electrokinetic chromatography

W.G.H.M. Muijselaar, M.A. Straten, van, H.A. Claessens, C.A.M.G. Cramers

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Diffusion coefficients in micellar electrokinetic chromatography (MEKC) were determined for a homologous series of alkylaryl ketones. Overall diffusion coefficients in micellar electrolyte systems were calculated from measured spatial peak variances, using the stopped migration method. From overall diffusion coefficients at two different surfactant concentrations, diffusion coefficients in the aqueous phase and in the micellar phase, respectively, were determined. Diffusion in the micellar phase was found to be approximately one order of magnitude lower than diffusion in the aqueous phase for the alkylaryl ketones. The influence of micellar diffusion on efficiency is treated theoretically. It is demonstrated that efficiency in MEKC strongly depends on the retention factor, especially for small values of the elution window. Under practical MEKC conditions, however, the influence of micellar diffusion is of minor importance. The determination of separation numbers in MEKC is evaluated and their use as parameter for the separation performance is discussed
Original languageEnglish
Pages (from-to)187-195
JournalJournal of Chromatography, A
Issue number1-2
Publication statusPublished - 1997


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