Determination of conjugated bile acids in human bile by isotachophoresis in a non-aqueous solvent using a.c. conductivity and UV detection

J.C. Reijenga; H.J.L.A. Slaats; F.M. Everaerts

doi:10.1016/S0021-9673(01)90821-0

Determination of conjugated bile acids in human bile by isotachophoresis in a non-aqueous solvent using a.c. conductivity and UV detection

J.C. Reijenga, H.J.L.A. Slaats, F.M. Everaerts

Chemical Engineering and Chemistry

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Abstract

A method for the determination of conjugated bile acids in human bile using isotachophoresis in 95% methanol is described. The leading ion is 0.01 M chloride, the counter ion is hydroxylamine at its pK value and the terminating ion is N-2- hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES). The sample preparation consists of C18-silica cartridge adsorption. Microlitre amounts of the methanol eluate are injected and analysed within 20 min in a 0.2 mm I.D. PTFE capillary. The sensitivity of the method is better than 50 ng of each of the conjugated bile acids using a.c. conductivity detection.

Original language	English
Pages (from-to)	85-89
Journal	Journal of Chromatography
Volume	267
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(01)90821-0
Publication status	Published - 1983

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title = "Determination of conjugated bile acids in human bile by isotachophoresis in a non-aqueous solvent using a.c. conductivity and UV detection",

abstract = "A method for the determination of conjugated bile acids in human bile using isotachophoresis in 95\% methanol is described. The leading ion is 0.01 M chloride, the counter ion is hydroxylamine at its pK value and the terminating ion is N-2- hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES). The sample preparation consists of C18-silica cartridge adsorption. Microlitre amounts of the methanol eluate are injected and analysed within 20 min in a 0.2 mm I.D. PTFE capillary. The sensitivity of the method is better than 50 ng of each of the conjugated bile acids using a.c. conductivity detection.",

author = "J.C. Reijenga and H.J.L.A. Slaats and F.M. Everaerts",

year = "1983",

doi = "10.1016/S0021-9673(01)90821-0",

language = "English",

volume = "267",

pages = "85--89",

journal = "Journal of Chromatography",

issn = "0021-9673",

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T1 - Determination of conjugated bile acids in human bile by isotachophoresis in a non-aqueous solvent using a.c. conductivity and UV detection

AU - Reijenga, J.C.

AU - Slaats, H.J.L.A.

AU - Everaerts, F.M.

PY - 1983

Y1 - 1983

N2 - A method for the determination of conjugated bile acids in human bile using isotachophoresis in 95% methanol is described. The leading ion is 0.01 M chloride, the counter ion is hydroxylamine at its pK value and the terminating ion is N-2- hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES). The sample preparation consists of C18-silica cartridge adsorption. Microlitre amounts of the methanol eluate are injected and analysed within 20 min in a 0.2 mm I.D. PTFE capillary. The sensitivity of the method is better than 50 ng of each of the conjugated bile acids using a.c. conductivity detection.

AB - A method for the determination of conjugated bile acids in human bile using isotachophoresis in 95% methanol is described. The leading ion is 0.01 M chloride, the counter ion is hydroxylamine at its pK value and the terminating ion is N-2- hydroxyethylpiperazine-N'-2-ethanesulphonic acid (HEPES). The sample preparation consists of C18-silica cartridge adsorption. Microlitre amounts of the methanol eluate are injected and analysed within 20 min in a 0.2 mm I.D. PTFE capillary. The sensitivity of the method is better than 50 ng of each of the conjugated bile acids using a.c. conductivity detection.

U2 - 10.1016/S0021-9673(01)90821-0

DO - 10.1016/S0021-9673(01)90821-0

M3 - Article

SN - 0021-9673

VL - 267

SP - 85

EP - 89

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1

ER -

Determination of conjugated bile acids in human bile by isotachophoresis in a non-aqueous solvent using a.c. conductivity and UV detection

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