Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR

D. Medici, de; L. Croci; S. Pasquale, di; A. Fiore; L. Toti

doi:10.1046/j.1472-765X.2001.01018.x

Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR

D. Medici, de, L. Croci, S. Pasquale, di, A. Fiore, L. Toti

Photonics and Semiconductor Nanophysics

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Aims: The objective of this study was to det. the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. Methods and Results: One hundred and forty-two mollusc samples were analyzed for the presence of viral HAV-RNA using RT-nested-PCR; pos. samples were then analyzed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were pos. for the presence of infectious virus. Conclusions: The results demonstrate the validity of the screening method (RT-nested-PCR) and the necessity of applying a method that is capable of detecting the presence of infectious HAV. Significance and Impact of the Study: The study demonstrates that in any case, to det. the safety for human consumption, the results of RT-nested-PCR must be confirmed with an integrated cell-culture PCR method.

Original language	English
Pages (from-to)	362-366
Journal	Letters in Applied Microbiology
Volume	33
Issue number	5
DOIs	https://doi.org/10.1046/j.1472-765X.2001.01018.x
Publication status	Published - 2001

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Cite this

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abstract = "Aims: The objective of this study was to det. the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. Methods and Results: One hundred and forty-two mollusc samples were analyzed for the presence of viral HAV-RNA using RT-nested-PCR; pos. samples were then analyzed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were pos. for the presence of infectious virus. Conclusions: The results demonstrate the validity of the screening method (RT-nested-PCR) and the necessity of applying a method that is capable of detecting the presence of infectious HAV. Significance and Impact of the Study: The study demonstrates that in any case, to det. the safety for human consumption, the results of RT-nested-PCR must be confirmed with an integrated cell-culture PCR method.",

author = "{Medici, de}, D. and L. Croci and {Pasquale, di}, S. and A. Fiore and L. Toti",

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T1 - Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR

AU - Medici, de, D.

AU - Croci, L.

AU - Pasquale, di, S.

AU - Fiore, A.

AU - Toti, L.

PY - 2001

Y1 - 2001

N2 - Aims: The objective of this study was to det. the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. Methods and Results: One hundred and forty-two mollusc samples were analyzed for the presence of viral HAV-RNA using RT-nested-PCR; pos. samples were then analyzed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were pos. for the presence of infectious virus. Conclusions: The results demonstrate the validity of the screening method (RT-nested-PCR) and the necessity of applying a method that is capable of detecting the presence of infectious HAV. Significance and Impact of the Study: The study demonstrates that in any case, to det. the safety for human consumption, the results of RT-nested-PCR must be confirmed with an integrated cell-culture PCR method.

AB - Aims: The objective of this study was to det. the presence of infectious hepatitis A virus (HAV) in molluscs naturally contaminated with viral HAV-RNA. Methods and Results: One hundred and forty-two mollusc samples were analyzed for the presence of viral HAV-RNA using RT-nested-PCR; pos. samples were then analyzed with an integrated method, cell-culture RT-PCR, to detect infectious virus. Viral HAV-RNA was detected in 34.5% of the samples while 12.7% of the total samples were pos. for the presence of infectious virus. Conclusions: The results demonstrate the validity of the screening method (RT-nested-PCR) and the necessity of applying a method that is capable of detecting the presence of infectious HAV. Significance and Impact of the Study: The study demonstrates that in any case, to det. the safety for human consumption, the results of RT-nested-PCR must be confirmed with an integrated cell-culture PCR method.

U2 - 10.1046/j.1472-765X.2001.01018.x

DO - 10.1046/j.1472-765X.2001.01018.x

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EP - 366

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

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ER -

Detecting the presence of infectious hepatitis A virus in molluscs positive to RT-nested-PCR

Abstract

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