Designing stable, hierarchical peptide fibers from block co-polypeptide sequences

Mark van Rijt, Adriano Ciaffoni, Alessandro Ianiro, Mohammad Moradi, A.L. Boyle, A. Kros, Heiner Friedrich, Nico Sommerdijk (Corresponding author), J.P. Patterson (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)
16 Downloads (Pure)

Abstract

Natural materials, such as collagen, can assemble with multiple levels of organization in solution. Achieving a similar degree of control over morphology, stability and hierarchical organization with equilibrium synthetic materials remains elusive. For the assembly of peptidic materials the process is controlled by a complex interplay between hydrophobic interactions, electrostatics and secondary structure formation. Consequently, fine tuning the thermodynamics and kinetics of assembly remains extremely challenging. Here, we synthesized a set of block co polypeptides with varying hydrophobicity and ability to form secondary structure. From this set we select a sequence with balanced interactions that results in the formation of high-aspect ratio thermodynamically favored nanotubes, stable between pH 2 and 12 and up to 80 °C. This stability permits their hierarchical assembly into bundled nanotube fibers by directing the pH and inducing complementary zwitterionic charge behavior. This block co-polypeptide design strategy, using defined sequences, provides a straightforward approach to creating complex hierarchical peptide-based assemblies with tunable interactions.

Translated title of the contributionDesigning stable, hierarchical peptide fibers from block co-polypeptide sequences
Original languageEnglish
Pages (from-to)9001-9008
Number of pages8
JournalChemical Science
Volume10
Issue number39
DOIs
Publication statusPublished - 7 Aug 2019

Fingerprint Dive into the research topics of 'Designing stable, hierarchical peptide fibers from block co-polypeptide sequences'. Together they form a unique fingerprint.

  • Cite this

    van Rijt, M., Ciaffoni, A., Ianiro, A., Moradi, M., Boyle, A. L., Kros, A., Friedrich, H., Sommerdijk, N., & Patterson, J. P. (2019). Designing stable, hierarchical peptide fibers from block co-polypeptide sequences. Chemical Science, 10(39), 9001-9008. https://doi.org/10.1039/c9sc00800d