Abstract
Electroporation technique is widely used in biotechnology and medicine for the transport of various molecules through the membranes of biological cells. Different mathematical models of electroporation have been proposed in the literature to study pore formation in plasma and nuclear membranes. These studies are mainly based on models using a single isolated cell with a canonical shape. In this work, a space–time (x,y,t) multiphysics model based on quasi-static Maxwell’s equations and nonlinear Smoluchowski’s equation has been developed to investigate the electroporation phenomenon induced by pulsed electric field in multicellular systems having irregularly shape. The dielectric dispersion of the cell compartments such as nuclear and plasmatic membranes, cytoplasm, nucleoplasm and external medium have been incorporated into the numerical algorithm, too. Moreover, the irregular cell shapes have been modeled by using the Gielis transformations.
| Original language | English |
|---|---|
| Article number | 37 |
| Number of pages | 17 |
| Journal | Electronics |
| Volume | 8 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
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Keywords
- Computational model
- Dielectric dispersion
- Nucleated biological cells
- Pulsed electric field
- Transmembrane voltage
Cite this
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Design of electroporation process in irregularly shaped multicellular systems. / Mescia, Luciano (Corresponding author); Chiapperino, Michele Alessandro; Bia, Pietro; Lamacchia, Claudio Maria; Gielis, Johan; Caratelli, Diego.
In: Electronics, Vol. 8, No. 1, 37, 01.01.2019.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Design of electroporation process in irregularly shaped multicellular systems
AU - Mescia, Luciano
AU - Chiapperino, Michele Alessandro
AU - Bia, Pietro
AU - Lamacchia, Claudio Maria
AU - Gielis, Johan
AU - Caratelli, Diego
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Electroporation technique is widely used in biotechnology and medicine for the transport of various molecules through the membranes of biological cells. Different mathematical models of electroporation have been proposed in the literature to study pore formation in plasma and nuclear membranes. These studies are mainly based on models using a single isolated cell with a canonical shape. In this work, a space–time (x,y,t) multiphysics model based on quasi-static Maxwell’s equations and nonlinear Smoluchowski’s equation has been developed to investigate the electroporation phenomenon induced by pulsed electric field in multicellular systems having irregularly shape. The dielectric dispersion of the cell compartments such as nuclear and plasmatic membranes, cytoplasm, nucleoplasm and external medium have been incorporated into the numerical algorithm, too. Moreover, the irregular cell shapes have been modeled by using the Gielis transformations.
AB - Electroporation technique is widely used in biotechnology and medicine for the transport of various molecules through the membranes of biological cells. Different mathematical models of electroporation have been proposed in the literature to study pore formation in plasma and nuclear membranes. These studies are mainly based on models using a single isolated cell with a canonical shape. In this work, a space–time (x,y,t) multiphysics model based on quasi-static Maxwell’s equations and nonlinear Smoluchowski’s equation has been developed to investigate the electroporation phenomenon induced by pulsed electric field in multicellular systems having irregularly shape. The dielectric dispersion of the cell compartments such as nuclear and plasmatic membranes, cytoplasm, nucleoplasm and external medium have been incorporated into the numerical algorithm, too. Moreover, the irregular cell shapes have been modeled by using the Gielis transformations.
KW - Computational model
KW - Dielectric dispersion
KW - Nucleated biological cells
KW - Pulsed electric field
KW - Transmembrane voltage
UR - http://www.scopus.com/inward/record.url?scp=85059454683&partnerID=8YFLogxK
U2 - 10.3390/electronics8010037
DO - 10.3390/electronics8010037
M3 - Article
AN - SCOPUS:85059454683
VL - 8
JO - Electronics
JF - Electronics
SN - 2079-9292
IS - 1
M1 - 37
ER -