Design of electroporation process in irregularly shaped multicellular systems

Luciano Mescia (Corresponding author), Michele Alessandro Chiapperino, Pietro Bia, Claudio Maria Lamacchia, Johan Gielis, Diego Caratelli

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)
18 Downloads (Pure)

Abstract

Electroporation technique is widely used in biotechnology and medicine for the transport of various molecules through the membranes of biological cells. Different mathematical models of electroporation have been proposed in the literature to study pore formation in plasma and nuclear membranes. These studies are mainly based on models using a single isolated cell with a canonical shape. In this work, a space–time (x,y,t) multiphysics model based on quasi-static Maxwell’s equations and nonlinear Smoluchowski’s equation has been developed to investigate the electroporation phenomenon induced by pulsed electric field in multicellular systems having irregularly shape. The dielectric dispersion of the cell compartments such as nuclear and plasmatic membranes, cytoplasm, nucleoplasm and external medium have been incorporated into the numerical algorithm, too. Moreover, the irregular cell shapes have been modeled by using the Gielis transformations.

Original languageEnglish
Article number37
Number of pages17
JournalElectronics
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

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Membranes
Maxwell equations
Biotechnology
Nonlinear equations
Medicine
Permittivity
Electric fields
Mathematical models
Plasmas
Molecules

Keywords

  • Computational model
  • Dielectric dispersion
  • Nucleated biological cells
  • Pulsed electric field
  • Transmembrane voltage

Cite this

Mescia, Luciano ; Chiapperino, Michele Alessandro ; Bia, Pietro ; Lamacchia, Claudio Maria ; Gielis, Johan ; Caratelli, Diego. / Design of electroporation process in irregularly shaped multicellular systems. In: Electronics. 2019 ; Vol. 8, No. 1.
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Design of electroporation process in irregularly shaped multicellular systems. / Mescia, Luciano (Corresponding author); Chiapperino, Michele Alessandro; Bia, Pietro; Lamacchia, Claudio Maria; Gielis, Johan; Caratelli, Diego.

In: Electronics, Vol. 8, No. 1, 37, 01.01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Design of electroporation process in irregularly shaped multicellular systems

AU - Mescia, Luciano

AU - Chiapperino, Michele Alessandro

AU - Bia, Pietro

AU - Lamacchia, Claudio Maria

AU - Gielis, Johan

AU - Caratelli, Diego

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AB - Electroporation technique is widely used in biotechnology and medicine for the transport of various molecules through the membranes of biological cells. Different mathematical models of electroporation have been proposed in the literature to study pore formation in plasma and nuclear membranes. These studies are mainly based on models using a single isolated cell with a canonical shape. In this work, a space–time (x,y,t) multiphysics model based on quasi-static Maxwell’s equations and nonlinear Smoluchowski’s equation has been developed to investigate the electroporation phenomenon induced by pulsed electric field in multicellular systems having irregularly shape. The dielectric dispersion of the cell compartments such as nuclear and plasmatic membranes, cytoplasm, nucleoplasm and external medium have been incorporated into the numerical algorithm, too. Moreover, the irregular cell shapes have been modeled by using the Gielis transformations.

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