Design of an affinity based cytokine release system to temporally control macrophage behavior

Emily Lurier, Anthal Smits, Carlijn V.C. Bouten, Kara L. Spiller

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

Statement of Purpose: Macrophages are key mediators in the inflammatory response to implanted biomaterials and their behavior ultimately determines the function and longevity of implants. Therefore, there is a need to modulate macrophage behavior in response to implanted biomaterials to promote healthy biomaterial-tissue integration. Here, we propose to use the biotin-streptavidin affinity system to modify scaffolds with immunomodulatory cytokines to promote the desired macrophage phenotype, which varies depending on the application. The bond between biotin and streptavidin has been assumed to be essentially covalent, however, biotinylated proteins exhibit reduced bond strength with streptavidin, leading to release of the biotinylated proteins. Additionally, in the presence of excess biotin, biotinylated proteins are displaced by free biotin due to the higher affinity between free biotin and streptavidin. Therefore, the biotin-streptavidin system holds potential to be used to impart controlled release functionality to any biomaterial that is amenable to biotinylation. The objective of this study was to investigate how conjugation parameters affect release of biotinylated macrophage-modulating proteins from biotinylated scaffolds. Gelatin sponges, a model biomaterial, were biotinylated with varying degrees of biotin, followed by attachment to biotinylated interleukin-4 (IL4) via streptavidin in order to promote the pro-healing "M2" macrophage phenotype. Additionally, two variants of avidin, streptavidin and captavidin, which have different affinities for biotin, were used to modulate the rate of release of protein over time.

Original languageEnglish
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
Place of PublicationMuttenz
PublisherSwiss Society for Biomaterials
Pages49
Number of pages1
ISBN (Electronic)9781510883901
Publication statusPublished - 1 Jan 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: 3 Apr 20196 Apr 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period3/04/196/04/19

Fingerprint

Behavior Control
Macrophages
Biotin
Streptavidin
Cytokines
Biocompatible Materials
Biomaterials
Proteins
Scaffolds (biology)
Biotinylation
Phenotype
Avidin
Porifera
Gelatin
Interleukin-4
Tissue

Cite this

Lurier, E., Smits, A., Bouten, C. V. C., & Spiller, K. L. (2019). Design of an affinity based cytokine release system to temporally control macrophage behavior. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (pp. 49). (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Muttenz: Swiss Society for Biomaterials.
Lurier, Emily ; Smits, Anthal ; Bouten, Carlijn V.C. ; Spiller, Kara L. / Design of an affinity based cytokine release system to temporally control macrophage behavior. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Muttenz : Swiss Society for Biomaterials, 2019. pp. 49 (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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Lurier, E, Smits, A, Bouten, CVC & Spiller, KL 2019, Design of an affinity based cytokine release system to temporally control macrophage behavior. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Swiss Society for Biomaterials, Muttenz, pp. 49, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 3/04/19.

Design of an affinity based cytokine release system to temporally control macrophage behavior. / Lurier, Emily; Smits, Anthal; Bouten, Carlijn V.C.; Spiller, Kara L.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Muttenz : Swiss Society for Biomaterials, 2019. p. 49 (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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M3 - Conference contribution

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Lurier E, Smits A, Bouten CVC, Spiller KL. Design of an affinity based cytokine release system to temporally control macrophage behavior. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Muttenz: Swiss Society for Biomaterials. 2019. p. 49. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).