Design and performance of a dark-field probe with confocal Raman spectroscopy for ophthalmic applications

Shuo Zhang, Roel J. Erckens, Franciscus H.M. Jongsma, Carroll A.B. Webers, Tos T.J.M. Berendschot (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

A dark-field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36-fold improvement in the signal-to-noise ratio at the phenylephrine HCl 1002 cm−1 peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable.
Original languageEnglish
Pages (from-to)1371-1375
Number of pages5
JournalJournal of Raman Spectroscopy
Volume52
Issue number7
DOIs
Publication statusPublished - Jul 2021

Keywords

  • Raman spectroscopy
  • aqueous humor
  • dark-field illumination
  • ocular drug

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