DEC-205 mediates antigen uptake and presentation by both resting and activated human plasmacytoid dendritic cells

J. Tel, D. Benitez-Ribas, S. Hoosemans, A. Cambi, G.J. Adema, Carl G Figdor, P.J. Tacken, I.J.M. de Vries

Research output: Contribution to journalArticleAcademicpeer-review

50 Citations (Scopus)

Abstract

DEC-205 is a type I C-type lectin receptor (CLR) that is expressed on various APC subsets and has been suggested to bind necrotic and apoptotic cells. Here we study DEC-205 characteristics in plasmacytoid DCs (pDCs) obtained from healthy individuals and assess its ability to mediate antigen presentation by isolating sufficient numbers of pDCs from apheresis material obtained from stage III/IV melanoma patients. The results demonstrate that DEC-205 is expressed on human pDCs. Internalization of DEC-205 after antibody ligation is clathrin- and dynamin-dependent as it is blocked by hypertonic shock or by inhibition of dynamin activity. Antibody targeting to DEC-205 does not affect TLR-induced expression levels of co-stimulatory and MHC molecules, but clearly impairs TLR-induced IFN-α secretion by 40%. We observed that TLR-mediated signaling increases DEC-205 expression levels without affecting receptor internalization. Moreover, human pDCs retained the capacity to present antigens via DEC-205 following TLR activation.

Original languageEnglish
Pages (from-to)1014-1023
Number of pages10
JournalEuropean Journal of Immunology
Volume41
Issue number4
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Keywords

  • Antigen Presentation
  • Antigens, CD
  • Cell Differentiation
  • Cell Proliferation
  • Cell Separation
  • Cells, Cultured
  • Dendritic Cells
  • Humans
  • Interferon-alpha
  • Lectins, C-Type
  • Receptors, Cell Surface
  • Toll-Like Receptors
  • Journal Article
  • Research Support, Non-U.S. Gov't

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