Cytostretch, an organ-on-chip platform

Nikolas Gaio, Berend van Meer, William Quirós Solano, Lambert Bergers, Anja van de Stolpe, Christine Mummery, Pasqualina M. Sarro, Ronald Dekker

Research output: Contribution to journalArticleAcademicpeer-review

34 Citations (Scopus)
103 Downloads (Pure)

Abstract

Organ-on-Chips (OOCs) are micro-fabricated devices which are used to culture cells in order to mimic functional units of human organs. The devices are designed to simulate the physiological environment of tissues in vivo. Cells in some types of OOCs can be stimulated in situ by electrical and/or mechanical actuators. These actuations can mimic physiological conditions in real tissue and may include fluid or air flow, or cyclic stretch and strain as they occur in the lung and heart. These conditions similarly affect cultured cells and may influence their ability to respond appropriately to physiological or pathological stimuli. To date, most focus has been on devices specifically designed to culture just one functional unit of a specific organ: Lung alveoli, kidney nephrons or blood vessels, for example. In contrast, the modular Cytostretch membrane platform described here allows OOCs to be customized to different OOC applications. The platform utilizes silicon-based micro-fabrication techniques that allow low-cost, high-volume manufacturing. We describe the platform concept and its modules developed to date. Membrane variants include membranes with (i) through-membrane pores that allow biological signaling molecules to pass between two different tissue compartments; (ii) a stretchable micro-electrode array for electrical monitoring and stimulation; (iii) micro-patterning to promote cell alignment; and (iv) strain gauges to measure changes in substrate stress. This paper presents the fabrication and the proof of functionality for each module of the Cytostretch membrane. The assessment of each additional module demonstrate that a wide range of OOCs can be achieved.

Original languageEnglish
Article number120
JournalMicromachines
Volume7
Issue number7
DOIs
Publication statusPublished - 14 Jul 2016

Keywords

  • Customizable
  • Cytostretch
  • Micro-electrode array
  • Micro-grooves
  • Modular
  • Organ-on-chip
  • Platform
  • Stem cells
  • Strain gauges
  • Through-membrane pores

Fingerprint

Dive into the research topics of 'Cytostretch, an organ-on-chip platform'. Together they form a unique fingerprint.

Cite this