Cross-correlation suppressed T1 and NOE experiments for protein side-chain 13CH2 groups

Changwen Jin, J.J. Prompers, R. Brüschweiler

    Research output: Contribution to journalArticleAcademicpeer-review

    11 Citations (Scopus)


    Relaxation measurements of side-chain 13CH2-groups of uniformly 13C labeled human ubiquitin were performed at 600 MHz and 800 MHz magnetic field strength at 30°C. Dipole-dipole cross-correlated relaxation effects in T1 experiments were suppressed by the combination of radio-frequency pulses and pulsed field gradients during the relaxation delay leading to monoexponential relaxation decays that allow a more accurate extraction of the 13C T1 relaxation times. Heteronuclear 1H-13C NOEs obtained by using different proton saturation schemes indicate that the influence of cross-correlation is small. The experimental T1 and NOE data were interpreted in a model-free way in terms of a generalized order parameter and an internal correlation time.
    Original languageEnglish
    Pages (from-to)241-247
    JournalJournal of Biomolecular NMR
    Issue number3
    Publication statusPublished - 2003


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