Control of oxidative stress in Jurkat cells as a model of leukemia treatment

S. Pshenichnikov; A. Omelyanchik; M. Efremova; M. Lunova; N. Gazatova; V. Malashchenko; O. Khaziakhmatova; L. Litvinova; N. Perov; L. Panina; D. Peddis; O. Lunov; V. Rodionova; K. Levada

doi:10.1016/j.jmmm.2020.167623

Control of oxidative stress in Jurkat cells as a model of leukemia treatment

S. Pshenichnikov, A. Omelyanchik, M. Efremova, M. Lunova, N. Gazatova, V. Malashchenko, O. Khaziakhmatova, L. Litvinova, N. Perov, L. Panina, D. Peddis, O. Lunov, V. Rodionova (Corresponding author), K. Levada

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

This paper describes proof-of-concept for antileukemia treatment based on the reactive oxygen species (ROS) induction supplemented with magnetic nanoparticles in the presence of moderate-intensity magnetic fields. The T-lymphoblastic leukemia cell line (Jurkat) was chosen as an in vitro model of acute lymphoblastic leukemia. The elevated ROS levels were generated by iron oxide magnetic nanoparticles. We found an enhanced cytotoxic effect of magnetic nanoparticles in the presence of a magnetic field on cancerous Jurkat cell line due to induced oxidative stress after cultivation in magnetic fields with an average strength of 0.26–0.36 T and the field spatial gradient up to 100 T/m. At the same conditions of nanoparticles intake, the viability of human peripheral blood mononuclear cells (model of healthy cells) was not inhibited. Thus, we demonstrated the feasibility of antileukemia treatment by magnetic nanoparticles controlled by a magnetic field.

Original language	English
Article number	167623
Number of pages	7
Journal	Journal of Magnetism and Magnetic Materials
Volume	523
DOIs	https://doi.org/10.1016/j.jmmm.2020.167623
Publication status	Published - 1 Apr 2021
Externally published	Yes

Bibliographical note

Funding Information:
The authors acknowledge the Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 – CZ.02.1.01/0.0/0.0/16_019/0000760). The study was supported by the Grant of the President of the Russian Federation for Leading Scientific Schools (NSh- HШ-2495.2020.7). This work was supported in part by the 5 top 100 Russian Academic Excellence Project at the Immanuel Kant Baltic Federal University. The authors are grateful to PhD student of IKBFU Egor Shunkin for the assistance in the realization of the experimental part of the project.

Funding

The authors acknowledge the Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 – CZ.02.1.01/0.0/0.0/16_019/0000760). The study was supported by the Grant of the President of the Russian Federation for Leading Scientific Schools (NSh- HШ-2495.2020.7). This work was supported in part by the 5 top 100 Russian Academic Excellence Project at the Immanuel Kant Baltic Federal University. The authors are grateful to PhD student of IKBFU Egor Shunkin for the assistance in the realization of the experimental part of the project.

Funders	Funder number
Immanuel Kant Baltic Federal University
Ministerstvo Školství, Mládeže a Tělovýchovy	SOLID21 – CZ.02.1.01/0.0/0.0/16_019/0000760

Keywords

Cytotoxicity
Jurkat
Leukemia
Magnetic fields
Magnetic nanoparticles
PBMC

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jmmm.2020.167623

Cite this

Pshenichnikov, S., Omelyanchik, A., Efremova, M., Lunova, M., Gazatova, N., Malashchenko, V., Khaziakhmatova, O., Litvinova, L., Perov, N., Panina, L., Peddis, D., Lunov, O., Rodionova, V., & Levada, K. (2021). Control of oxidative stress in Jurkat cells as a model of leukemia treatment. Journal of Magnetism and Magnetic Materials, 523, Article 167623. https://doi.org/10.1016/j.jmmm.2020.167623

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abstract = "This paper describes proof-of-concept for antileukemia treatment based on the reactive oxygen species (ROS) induction supplemented with magnetic nanoparticles in the presence of moderate-intensity magnetic fields. The T-lymphoblastic leukemia cell line (Jurkat) was chosen as an in vitro model of acute lymphoblastic leukemia. The elevated ROS levels were generated by iron oxide magnetic nanoparticles. We found an enhanced cytotoxic effect of magnetic nanoparticles in the presence of a magnetic field on cancerous Jurkat cell line due to induced oxidative stress after cultivation in magnetic fields with an average strength of 0.26–0.36 T and the field spatial gradient up to 100 T/m. At the same conditions of nanoparticles intake, the viability of human peripheral blood mononuclear cells (model of healthy cells) was not inhibited. Thus, we demonstrated the feasibility of antileukemia treatment by magnetic nanoparticles controlled by a magnetic field.",

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note = "Funding Information: The authors acknowledge the Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 – CZ.02.1.01/0.0/0.0/16_019/0000760). The study was supported by the Grant of the President of the Russian Federation for Leading Scientific Schools (NSh- HШ-2495.2020.7). This work was supported in part by the 5 top 100 Russian Academic Excellence Project at the Immanuel Kant Baltic Federal University. The authors are grateful to PhD student of IKBFU Egor Shunkin for the assistance in the realization of the experimental part of the project. ",

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Pshenichnikov, S, Omelyanchik, A, Efremova, M, Lunova, M, Gazatova, N, Malashchenko, V, Khaziakhmatova, O, Litvinova, L, Perov, N, Panina, L, Peddis, D, Lunov, O, Rodionova, V & Levada, K 2021, 'Control of oxidative stress in Jurkat cells as a model of leukemia treatment', Journal of Magnetism and Magnetic Materials, vol. 523, 167623. https://doi.org/10.1016/j.jmmm.2020.167623

TY - JOUR

T1 - Control of oxidative stress in Jurkat cells as a model of leukemia treatment

AU - Pshenichnikov, S.

AU - Omelyanchik, A.

AU - Efremova, M.

AU - Lunova, M.

AU - Gazatova, N.

AU - Malashchenko, V.

AU - Khaziakhmatova, O.

AU - Litvinova, L.

AU - Perov, N.

AU - Panina, L.

AU - Peddis, D.

AU - Lunov, O.

AU - Rodionova, V.

AU - Levada, K.

N1 - Funding Information: The authors acknowledge the Operational Programme Research, Development and Education financed by European Structural and Investment Funds and the Czech Ministry of Education, Youth and Sports (Project No. SOLID21 – CZ.02.1.01/0.0/0.0/16_019/0000760). The study was supported by the Grant of the President of the Russian Federation for Leading Scientific Schools (NSh- HШ-2495.2020.7). This work was supported in part by the 5 top 100 Russian Academic Excellence Project at the Immanuel Kant Baltic Federal University. The authors are grateful to PhD student of IKBFU Egor Shunkin for the assistance in the realization of the experimental part of the project.

PY - 2021/4/1

Y1 - 2021/4/1

N2 - This paper describes proof-of-concept for antileukemia treatment based on the reactive oxygen species (ROS) induction supplemented with magnetic nanoparticles in the presence of moderate-intensity magnetic fields. The T-lymphoblastic leukemia cell line (Jurkat) was chosen as an in vitro model of acute lymphoblastic leukemia. The elevated ROS levels were generated by iron oxide magnetic nanoparticles. We found an enhanced cytotoxic effect of magnetic nanoparticles in the presence of a magnetic field on cancerous Jurkat cell line due to induced oxidative stress after cultivation in magnetic fields with an average strength of 0.26–0.36 T and the field spatial gradient up to 100 T/m. At the same conditions of nanoparticles intake, the viability of human peripheral blood mononuclear cells (model of healthy cells) was not inhibited. Thus, we demonstrated the feasibility of antileukemia treatment by magnetic nanoparticles controlled by a magnetic field.

AB - This paper describes proof-of-concept for antileukemia treatment based on the reactive oxygen species (ROS) induction supplemented with magnetic nanoparticles in the presence of moderate-intensity magnetic fields. The T-lymphoblastic leukemia cell line (Jurkat) was chosen as an in vitro model of acute lymphoblastic leukemia. The elevated ROS levels were generated by iron oxide magnetic nanoparticles. We found an enhanced cytotoxic effect of magnetic nanoparticles in the presence of a magnetic field on cancerous Jurkat cell line due to induced oxidative stress after cultivation in magnetic fields with an average strength of 0.26–0.36 T and the field spatial gradient up to 100 T/m. At the same conditions of nanoparticles intake, the viability of human peripheral blood mononuclear cells (model of healthy cells) was not inhibited. Thus, we demonstrated the feasibility of antileukemia treatment by magnetic nanoparticles controlled by a magnetic field.

KW - Cytotoxicity

KW - Jurkat

KW - Leukemia

KW - Magnetic fields

KW - Magnetic nanoparticles

KW - PBMC

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U2 - 10.1016/j.jmmm.2020.167623

DO - 10.1016/j.jmmm.2020.167623

M3 - Article

AN - SCOPUS:85099211143

SN - 0304-8853

VL - 523

JO - Journal of Magnetism and Magnetic Materials

JF - Journal of Magnetism and Magnetic Materials

M1 - 167623

ER -

Control of oxidative stress in Jurkat cells as a model of leukemia treatment

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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