Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds

S. Hofmann, H. Hagenmüller, A.M. Koch, R. Müller, G. Vunjak-Novakovic, D.L. Kaplan, H.P. Merkle, L. Meinel

Research output: Contribution to journalArticleAcademicpeer-review

276 Citations (Scopus)
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Abstract

Natural bone consists of cortical and trabecular morphologies, the latter having variable pore sizes. This study aims at engineering different bone-like structures using scaffolds with small pores (112-224 µm) in diameter on one side and large pores (400-500 µm) on the other, while keeping scaffold porosities constant among groups. We hypothesized that tissue engineered bone-like structure resulting from silk fibroin (SF) implants is pre-determined by the scaffolds' geometry. To test this hypothesis, SF scaffolds with different pore diameters were prepared and seeded with human mesenchymal stem cells (hMSC). As compared to static seeding, dynamic cell seeding in spinner flasks resulted in equal cell viability and proliferation, and better cell distribution throughout the scaffold as visualized by histology and confocal microscopy, and was, therefore, selected for subsequent differentiation studies. Differentiation of hMSC in osteogenic cell culture medium in spinner flasks for 3 and 5 weeks resulted in increased alkaline phosphatase activity and calcium deposition when compared to control medium. Micro-computed tomography (µCT) detailed the pore structures of the newly formed tissue and suggested that the structure of tissue-engineered bone was controlled by the underlying scaffold geometry. © 2006 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)1152-1162
Number of pages11
JournalBiomaterials
Volume28
Issue number6
DOIs
Publication statusPublished - 2007
Externally publishedYes

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