Continuous biomarker monitoring by particle mobility sensing with single molecule resolution

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Abstract

Healthcare is in demand of technologies for real-Time sensing in order to continuously guard the state of patients. Here we present biomarker-monitoring based on the sensing of particle mobility, a concept wherein particles are coupled to a substrate via a flexible molecular tether, with both the particles and substrate provided with affinity molecules for effectuating specific and reversible interactions. Single-molecular binding and unbinding events modulate the Brownian particle motion and the state changes are recorded using optical scattering microscopy. The technology is demonstrated with DNA and protein as model biomarkers, in buffer and in blood plasma, showing sensitivity to picomolar and nanomolar concentrations. The sensing principle is direct and self-contained, without consuming or producing any reactants. With its basis in reversible interactions and single-molecule resolution, we envisage that the presented technology will enable biosensors for continuous biomarker monitoring with high sensitivity, specificity, and accuracy.

Original languageEnglish
Article number2541
Number of pages10
JournalNature Communications
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

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biomarkers
Biomarkers
Technology
Molecules
Monitoring
blood plasma
molecules
sensitivity
particle motion
Biosensing Techniques
Substrates
bioinstrumentation
Biosensors
affinity
Microscopy
Microscopic examination
Buffers
Blood
deoxyribonucleic acid
buffers

Cite this

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