Confocal Raman spectroscopy: Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo

Christian J.F. Bertens (Corresponding author), Shuo Zhang, Roel J. Erckens, Frank J.H.M. van den Biggelaar, Tos T.J.M. Berendschot, Carroll A.B. Webers, Rudy M.M.A. Nuijts, Marlies Gijs

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Current information about the pharmacokinetics of an ocular drug can only be achieved by invasive sampling. However, confocal Raman spectroscopy bears the potential to quantify drug concentrations non-invasively. In this project, we evaluated the detection and quantification of ocular ketorolac tromethamine levels with confocal Raman spectroscopy after topical administration.

Confocal Raman spectroscopy and high-performance liquid chromatography (HPLC) were compared in terms of sensitivity of detection. Enucleated pig eyes were treated with different concentrations of ketorolac. Hereafter, ketorolac concentrations in the aqueous humor of pig eyes were analyzed by confocal Raman spectroscopy and HPLC.

Subsequently, twelve rabbits were treated with Acular™ for four weeks. At several time points, ketorolac concentrations in aqueous humor of the rabbits were measured by confocal Raman spectroscopy followed by drawing an aqueous humor sample for HPLC analysis.

In ketorolac treated pig eyes, both ex vivo Raman spectroscopy as well as HPLC were able to detect ketorolac in a broad concentration range. However, in vivo confocal Raman spectroscopy in rabbits was unable to detect ketorolac in contrast to HPLC.

To conclude, confocal Raman spectroscopy has the capacity to detect ketorolac tromethamine in vitro, but currently lacks sensitivity for in vivo detection.
Original languageEnglish
Article number118641
Number of pages9
JournalInternational Journal of Pharmaceutics
Volume570
DOIs
Publication statusPublished - 30 Oct 2019

Keywords

  • Raman spectroscopy
  • High-performance liquid chromatography
  • In vivo drug detection
  • Ketorolac tromethamine
  • Non-invasive drug detection

Cite this

Bertens, Christian J.F. ; Zhang, Shuo ; Erckens, Roel J. ; van den Biggelaar, Frank J.H.M. ; Berendschot, Tos T.J.M. ; Webers, Carroll A.B. ; Nuijts, Rudy M.M.A. ; Gijs, Marlies. / Confocal Raman spectroscopy : Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo. In: International Journal of Pharmaceutics. 2019 ; Vol. 570.
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abstract = "Current information about the pharmacokinetics of an ocular drug can only be achieved by invasive sampling. However, confocal Raman spectroscopy bears the potential to quantify drug concentrations non-invasively. In this project, we evaluated the detection and quantification of ocular ketorolac tromethamine levels with confocal Raman spectroscopy after topical administration.Confocal Raman spectroscopy and high-performance liquid chromatography (HPLC) were compared in terms of sensitivity of detection. Enucleated pig eyes were treated with different concentrations of ketorolac. Hereafter, ketorolac concentrations in the aqueous humor of pig eyes were analyzed by confocal Raman spectroscopy and HPLC.Subsequently, twelve rabbits were treated with Acular™ for four weeks. At several time points, ketorolac concentrations in aqueous humor of the rabbits were measured by confocal Raman spectroscopy followed by drawing an aqueous humor sample for HPLC analysis.In ketorolac treated pig eyes, both ex vivo Raman spectroscopy as well as HPLC were able to detect ketorolac in a broad concentration range. However, in vivo confocal Raman spectroscopy in rabbits was unable to detect ketorolac in contrast to HPLC.To conclude, confocal Raman spectroscopy has the capacity to detect ketorolac tromethamine in vitro, but currently lacks sensitivity for in vivo detection.",
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Confocal Raman spectroscopy : Evaluation of a non-invasive technique for the detection of topically applied ketorolac tromethamine in vitro and in vivo. / Bertens, Christian J.F. (Corresponding author); Zhang, Shuo; Erckens, Roel J.; van den Biggelaar, Frank J.H.M.; Berendschot, Tos T.J.M.; Webers, Carroll A.B.; Nuijts, Rudy M.M.A.; Gijs, Marlies.

In: International Journal of Pharmaceutics, Vol. 570, 118641, 30.10.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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