Conductometric detection during isotachophoresis

F.M. Everaerts, P. Prose, T.P.E.M. Verheggen

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It is beyond the scope of this paper to deal with theoretic aspects of isotachophoresis or other electrophoretic methods. This can easily be found in scientific literature,o -3) where several authors already give extensive explanations about principles and theory of, for example, isotachophoresis. Some data is given of isotachophoretic separation of fruit juices. In the analyses of these juices special attention was paid for components commonly present in natural juices. By the application of four operational systems, Le. systems (electrolytic) which can be applied for isotachophoretic analyses, at four different pHs, these components were determined qualitatively and quantitatively of their presence. In most of the juices at least ten more components could be visualized, but not yet determined. The detection of these components was possible by the combination of the two high resolution detectors: the UV detector and the recently developed conductometric detector. In order to show how the qualitative information can be deduced from isotachopherograms, data of nucleotides and some related components are shown, collected in four operational systems (pH 7, 6, 5,4).
Original languageEnglish
Pages (from-to)721-726
JournalProtides of the Biological Fluids
Publication statusPublished - 1975


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