Computational simulation of migration and dispersion in free capillary zone electrophoresis, II: Results of simulation and comparison with measurements

J.C. Reijenga, E. Kenndler

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Abstract

The results of the simulation of electropherograms for CZE obtained by the instrumental simulator described in Part I are presented as functions of various experimental parameters. The electropherograms, demonstrating the effects of the injection zone length and sample composition on peak broadening, of the potential on migration and efficiency, of minute changes in pH on the resolution, of the ionic strength of the buffer on the separation selectivity and of the co-ion of the background electrolyte on peak shape, are simulated within a few seconds. A comparison was made between simulated electropherograms and those obtained by measurements with real equipment.
Original languageEnglish
Pages (from-to)417-426
JournalJournal of Chromatography, A
Volume659
Issue number2
DOIs
Publication statusPublished - 1994

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