Combined enzymatic degradation of proteoglycans and collagen significantly alters intratissue strains in articular cartilage during cyclic compression

Maria Ioana Pastrama (Corresponding author), Ana Caxaido Ortiz, Lianne Zevenbergen, Nele Famaey, Willy Gsell, Corey P. Neu, Uwe Himmelreich, Ilse Jonkers

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Abstract

As degenerative joint diseases such as osteoarthritis (OA) progress, the matrix constituents, particularly collagen fibrils and proteoglycans, become damaged, therefore deteriorating the tissue's mechanical properties. This study aims to further the understanding of the effect of degradation of the different cartilage constituents on the mechanical loading environment in early stage OA. To this end, intact, collagen- and proteoglycan-depleted cartilage plugs were cyclically loaded in axial compression using an experimental model simulating in vivo cartilage-on-cartilage contact conditions in a micro-MRI scanner. Depletion of collagen and proteoglycans was achieved through enzymatic degradation with collagenase and chondroitinase ABC, respectively. Using a displacement-encoded imaging sequence (DENSE), strains were computed and compared in intact and degraded samples. The results revealed that, while degradation with one or the other enzyme had little effect on the contact strains, degradation with a combination of both enzymes caused an increase in the means and variance of the transverse, axial and shear strains, particularly in the superficial zone of the cartilage. This effect indicates that the balance between cartilage matrix constituents plays an essential role in maintaining the mechanical properties of the tissue, and a disturbance in this balance leads to a decrease of the load bearing capacity associated with degenerative joint diseases such as OA.

Original languageEnglish
Pages (from-to)383-394
Number of pages12
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume98
DOIs
Publication statusPublished - 1 Oct 2019

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Cartilage
Proteoglycans
Collagen
Degradation
Enzymes
Chondroitin ABC Lyase
Tissue
Mechanical properties
Axial compression
Shear strain
Collagenases
Bearing capacity
Magnetic resonance imaging
Loads (forces)
Imaging techniques

Keywords

  • Articular cartilage
  • Displacement-encoded micro-MRI
  • Dynamic compression
  • Enzymatic degradation
  • Strain analysis

Cite this

Pastrama, Maria Ioana ; Ortiz, Ana Caxaido ; Zevenbergen, Lianne ; Famaey, Nele ; Gsell, Willy ; Neu, Corey P. ; Himmelreich, Uwe ; Jonkers, Ilse. / Combined enzymatic degradation of proteoglycans and collagen significantly alters intratissue strains in articular cartilage during cyclic compression. In: Journal of the Mechanical Behavior of Biomedical Materials. 2019 ; Vol. 98. pp. 383-394.
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abstract = "As degenerative joint diseases such as osteoarthritis (OA) progress, the matrix constituents, particularly collagen fibrils and proteoglycans, become damaged, therefore deteriorating the tissue's mechanical properties. This study aims to further the understanding of the effect of degradation of the different cartilage constituents on the mechanical loading environment in early stage OA. To this end, intact, collagen- and proteoglycan-depleted cartilage plugs were cyclically loaded in axial compression using an experimental model simulating in vivo cartilage-on-cartilage contact conditions in a micro-MRI scanner. Depletion of collagen and proteoglycans was achieved through enzymatic degradation with collagenase and chondroitinase ABC, respectively. Using a displacement-encoded imaging sequence (DENSE), strains were computed and compared in intact and degraded samples. The results revealed that, while degradation with one or the other enzyme had little effect on the contact strains, degradation with a combination of both enzymes caused an increase in the means and variance of the transverse, axial and shear strains, particularly in the superficial zone of the cartilage. This effect indicates that the balance between cartilage matrix constituents plays an essential role in maintaining the mechanical properties of the tissue, and a disturbance in this balance leads to a decrease of the load bearing capacity associated with degenerative joint diseases such as OA.",
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Combined enzymatic degradation of proteoglycans and collagen significantly alters intratissue strains in articular cartilage during cyclic compression. / Pastrama, Maria Ioana (Corresponding author); Ortiz, Ana Caxaido; Zevenbergen, Lianne; Famaey, Nele; Gsell, Willy; Neu, Corey P.; Himmelreich, Uwe; Jonkers, Ilse.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 98, 01.10.2019, p. 383-394.

Research output: Contribution to journalArticleAcademicpeer-review

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