Column liquid chromotography of tricyclic antidepressants

J.H.M. van den Berg, H.J.J.M. de Ruwe, R.S. Deelder, Th.A. Plomp

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

A column liquid chromatographic system for the analysis of tricyclic antidepressants in serum is described. A high separation efficiency can be obtained with a mixture of ethyl acetate, n-hexane and methylamine as eluent on a silica gel column. The retention is easily regulated by varying the concentration of n-hexane, the modifier methylamine and the water content of the ethyl acetate. Examples are given of separations of test mixtures of tricyclic antidepressants and of some of these drugs in serum. UV detection permits determinations down to the 10-ng level in serum.
Original languageEnglish
Pages (from-to)431-436
JournalJournal of Chromatography
Volume138
Issue number2
DOIs
Publication statusPublished - 1977

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Tricyclic Antidepressive Agents
Silica Gel
Liquids
Serum
Water content
Systems Analysis
Liquid Chromatography
Water
ethyl acetate
n-hexane
methylamine

Cite this

van den Berg, J. H. M., de Ruwe, H. J. J. M., Deelder, R. S., & Plomp, T. A. (1977). Column liquid chromotography of tricyclic antidepressants. Journal of Chromatography, 138(2), 431-436. https://doi.org/10.1016/S0021-9673(00)94978-1
van den Berg, J.H.M. ; de Ruwe, H.J.J.M. ; Deelder, R.S. ; Plomp, Th.A. / Column liquid chromotography of tricyclic antidepressants. In: Journal of Chromatography. 1977 ; Vol. 138, No. 2. pp. 431-436.
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van den Berg, JHM, de Ruwe, HJJM, Deelder, RS & Plomp, TA 1977, 'Column liquid chromotography of tricyclic antidepressants', Journal of Chromatography, vol. 138, no. 2, pp. 431-436. https://doi.org/10.1016/S0021-9673(00)94978-1

Column liquid chromotography of tricyclic antidepressants. / van den Berg, J.H.M.; de Ruwe, H.J.J.M.; Deelder, R.S.; Plomp, Th.A.

In: Journal of Chromatography, Vol. 138, No. 2, 1977, p. 431-436.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van den Berg, J.H.M.

AU - de Ruwe, H.J.J.M.

AU - Deelder, R.S.

AU - Plomp, Th.A.

PY - 1977

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N2 - A column liquid chromatographic system for the analysis of tricyclic antidepressants in serum is described. A high separation efficiency can be obtained with a mixture of ethyl acetate, n-hexane and methylamine as eluent on a silica gel column. The retention is easily regulated by varying the concentration of n-hexane, the modifier methylamine and the water content of the ethyl acetate. Examples are given of separations of test mixtures of tricyclic antidepressants and of some of these drugs in serum. UV detection permits determinations down to the 10-ng level in serum.

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DO - 10.1016/S0021-9673(00)94978-1

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JO - Journal of Chromatography

JF - Journal of Chromatography

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