Coiled coil driven membrane fusion between cyclodextrin vesicles and liposomes

Frank Versluis, Jens Voskuhl, Jan Vos, Heiner Friedrich, Bart Jan Ravoo, Paul H.H. Bomans, Marc C.A. Stuart, Nico A.J.M. Sommerdijk, Alexander Kros

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)
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Abstract

Controlled fusion events between natural membranes composed of phospholipids with synthetic unnatural membranes will yield valuable fundamental information on the mechanism of membrane fusion. Here, fusion between vastly different phospholipid liposomes and cyclodextrin amphiphile based vesicles (CDVs) controlled by a pair of coiled coil forming lipidated peptides was investigated. Fusion events were characterized using lipid and content mixing assays and the resulting hybrid assemblies were characterized with cryo-TEM imaging. The secondary/quaternary structure of the lipidated peptides at the membrane interface was studied using circular dichroism spectroscopy. This is the first example of targeted fusion between natural and non-natural bilayer membranes and the in situ formation of hybrid CDV-liposome structures is of interest as it yields fundamental information about the mechanism through which fusion proceeds.

Original languageEnglish
Pages (from-to)9746-9751
Number of pages6
JournalSoft Matter
Volume10
Issue number48
DOIs
Publication statusPublished - 28 Dec 2014

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