Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films

M. Savoini; Xiaofei Wu; M. Celebrano; J. Ziegler; P. Biagioni; S.C.J. Meskers; L. Duo; B. Hecht; M. Finazzi

doi:10.1021/ja209916y

Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films

M. Savoini, Xiaofei Wu, M. Celebrano, J. Ziegler, P. Biagioni, S.C.J. Meskers, L. Duo, B. Hecht, M. Finazzi

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Abstract

Two-photon fluorescence scanning confocal microscopy sensitive to circular dichroism with a diffraction-limited resolution well below 500 nm is demonstrated. With this method, the spatial variation of the circular dichroism of thermally annealed chiral polyfluorene thin films has been imaged. We observed circular dichroism associated with submicrometer-sized domains showing helicoidally twisted macromolecular organization. Domains with opposite chiroptical properties, corresponding to left- or right-handed molecular organization, coexist in the film. Our results are consistent with those obtained by one-photon imaging and illustrate the potential of two-photon imaging for use in studying helical macromolecular organization.

Original language	English
Pages (from-to)	5832-5835
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	134
Issue number	13
DOIs	https://doi.org/10.1021/ja209916y
Publication status	Published - 2012

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title = "Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films",

abstract = "Two-photon fluorescence scanning confocal microscopy sensitive to circular dichroism with a diffraction-limited resolution well below 500 nm is demonstrated. With this method, the spatial variation of the circular dichroism of thermally annealed chiral polyfluorene thin films has been imaged. We observed circular dichroism associated with submicrometer-sized domains showing helicoidally twisted macromolecular organization. Domains with opposite chiroptical properties, corresponding to left- or right-handed molecular organization, coexist in the film. Our results are consistent with those obtained by one-photon imaging and illustrate the potential of two-photon imaging for use in studying helical macromolecular organization.",

author = "M. Savoini and Xiaofei Wu and M. Celebrano and J. Ziegler and P. Biagioni and S.C.J. Meskers and L. Duo and B. Hecht and M. Finazzi",

year = "2012",

doi = "10.1021/ja209916y",

language = "English",

volume = "134",

pages = "5832--5835",

journal = "Journal of the American Chemical Society",

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T1 - Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films

AU - Savoini, M.

AU - Wu, Xiaofei

AU - Celebrano, M.

AU - Ziegler, J.

AU - Biagioni, P.

AU - Meskers, S.C.J.

AU - Duo, L.

AU - Hecht, B.

AU - Finazzi, M.

PY - 2012

Y1 - 2012

N2 - Two-photon fluorescence scanning confocal microscopy sensitive to circular dichroism with a diffraction-limited resolution well below 500 nm is demonstrated. With this method, the spatial variation of the circular dichroism of thermally annealed chiral polyfluorene thin films has been imaged. We observed circular dichroism associated with submicrometer-sized domains showing helicoidally twisted macromolecular organization. Domains with opposite chiroptical properties, corresponding to left- or right-handed molecular organization, coexist in the film. Our results are consistent with those obtained by one-photon imaging and illustrate the potential of two-photon imaging for use in studying helical macromolecular organization.

AB - Two-photon fluorescence scanning confocal microscopy sensitive to circular dichroism with a diffraction-limited resolution well below 500 nm is demonstrated. With this method, the spatial variation of the circular dichroism of thermally annealed chiral polyfluorene thin films has been imaged. We observed circular dichroism associated with submicrometer-sized domains showing helicoidally twisted macromolecular organization. Domains with opposite chiroptical properties, corresponding to left- or right-handed molecular organization, coexist in the film. Our results are consistent with those obtained by one-photon imaging and illustrate the potential of two-photon imaging for use in studying helical macromolecular organization.

U2 - 10.1021/ja209916y

DO - 10.1021/ja209916y

M3 - Article

C2 - 22413739

SN - 0002-7863

VL - 134

SP - 5832

EP - 5835

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 13

ER -

Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films

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