Chromatographic properties of reversed-phase stationary phases under pressure- and electro-driven conditions

J. Jiskra, C.A.M.G. Cramers, M. Byelik, H.A. Claessens

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20 Citations (Scopus)

Abstract

Seven different reversed-phase (RP) stationary phases were examined under high-performance liquid chromatographic (pressure-driven, HPLC), and capillary electrochromatographic (electro-driven, CEC) conditions. Characterization of the stationary phases was performed following well-established test procedures providing a number of distinct column descriptors: hydrophobicity, hydrophobic selectivity and silanol activity. These parameters were used to describe the behavior of the RP-columns under both HPLC and CEC conditions. It is shown that chromatographic characteristics of porous RP-phases greatly depend on the mode of operation. By contrast, column descriptors of a non-porous viz. solid RP-phase material hardly differed for HPLC and CEC conditions.
Original languageEnglish
Pages (from-to)121-135
JournalJournal of Chromatography, A
Volume862
Issue number2
DOIs
Publication statusPublished - 1999

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