TY - JOUR
T1 - Chromatographic properties of reversed-phase stationary phases under pressure- and electro-driven conditions
AU - Jiskra, J.
AU - Cramers, C.A.M.G.
AU - Byelik, M.
AU - Claessens, H.A.
PY - 1999
Y1 - 1999
N2 - Seven different reversed-phase (RP) stationary phases were examined under high-performance liquid chromatographic (pressure-driven, HPLC), and capillary electrochromatographic (electro-driven, CEC) conditions. Characterization of the stationary phases was performed following well-established test procedures providing a number of distinct column descriptors: hydrophobicity, hydrophobic selectivity and silanol activity. These parameters were used to describe the behavior of the RP-columns under both HPLC and CEC conditions. It is shown that chromatographic characteristics of porous RP-phases greatly depend on the mode of operation. By contrast, column descriptors of a non-porous viz. solid RP-phase material hardly differed for HPLC and CEC conditions.
AB - Seven different reversed-phase (RP) stationary phases were examined under high-performance liquid chromatographic (pressure-driven, HPLC), and capillary electrochromatographic (electro-driven, CEC) conditions. Characterization of the stationary phases was performed following well-established test procedures providing a number of distinct column descriptors: hydrophobicity, hydrophobic selectivity and silanol activity. These parameters were used to describe the behavior of the RP-columns under both HPLC and CEC conditions. It is shown that chromatographic characteristics of porous RP-phases greatly depend on the mode of operation. By contrast, column descriptors of a non-porous viz. solid RP-phase material hardly differed for HPLC and CEC conditions.
U2 - 10.1016/S0021-9673(99)00933-4
DO - 10.1016/S0021-9673(99)00933-4
M3 - Article
SN - 0021-9673
VL - 862
SP - 121
EP - 135
JO - Journal of Chromatography, A
JF - Journal of Chromatography, A
IS - 2
ER -