Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids

Jonathan P. Wojciechowski; Adam D. Martin; Alexander F. Mason; Christopher M. Fife; Sharon M. Sagnella; Maria Kavallaris; Pall Thordarson

doi:10.1002/cplu.201600464

Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids

Jonathan P. Wojciechowski, Adam D. Martin, Alexander F. Mason, Christopher M. Fife, Sharon M. Sagnella, Maria Kavallaris, Pall Thordarson

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

Two peptide-derived low-molecular-weight gelators bearing different capping groups, 9-fluorenylmethyloxycarbonyl (Fmoc) and phenothiazine, were synthesized and their gel networks were characterized. The variation of the N-terminal capping group affects the viability of these hydrogels as a three-dimensional cell culture for multicellular tumor spheroids. These results indicate that the phenothiazine capping group is a more biocompatible alternative to the widely used Fmoc moiety.

Original language	English
Pages (from-to)	383-389
Number of pages	7
Journal	ChemPlusChem
Volume	82
Issue number	3
DOIs	https://doi.org/10.1002/cplu.201600464
Publication status	Published - 1 Mar 2017
Externally published	Yes

Funding

We thank the Mark Wainwright Analytical Centre (UNSW) for access to instruments. We acknowledge the Australian Research Council for an ARC Centre of Excellence grant (CE14010036) to P.T and M.K, a Discovery Project grant (DP130101512), and a Future Fellowship (FT120100101) to P.T, and the National Health and Medical Research Council for a Senior Research Fellowship to M. K. and Dementia Research Development Fellowship (APP1106751) to A.D.M.

Keywords

cytotoxicity
gels
peptides
self-assembly
tumor spheroids

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abstract = "Two peptide-derived low-molecular-weight gelators bearing different capping groups, 9-fluorenylmethyloxycarbonyl (Fmoc) and phenothiazine, were synthesized and their gel networks were characterized. The variation of the N-terminal capping group affects the viability of these hydrogels as a three-dimensional cell culture for multicellular tumor spheroids. These results indicate that the phenothiazine capping group is a more biocompatible alternative to the widely used Fmoc moiety.",

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AU - Fife, Christopher M.

AU - Sagnella, Sharon M.

AU - Kavallaris, Maria

AU - Thordarson, Pall

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KW - peptides

KW - self-assembly

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Choice of capping group in tripeptide hydrogels influences viability in the three-dimensional cell culture of tumor spheroids

Abstract

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Keywords

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