Characterization of Polar and Non-Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia

Ting Hun Lee; Chia Hau Lee; Nurul Alia Azmi; Supparmaniam Kavita; Syieluing Wong; Mansour Znati; Hichem Ben Jannet

doi:10.1002/cbdv.201900419

Characterization of Polar and Non-Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia

Ting Hun Lee (Corresponding author), Chia Hau Lee, Nurul Alia Azmi, Supparmaniam Kavita, Syieluing Wong, Mansour Znati, Hichem Ben Jannet

Research output: Contribution to journal › Article › Academic › peer-review

31 Citations (Scopus)

Abstract

This work investigated the polar (PC: protein, amino acid and metabolite) and non-polar (NPC: fatty acid) compounds and bioactivity characteristics of the EBN harvested from the state of Johor in Malaysia. The electrophoretic gels exhibited 15 protein bands (16–173 kD) with unique protein profile. Amino acids analysis by AccQ⋅Tag method revealed 18 types of amino acids in EBN. Metabolite profiling was performed using High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometer (HPLC-QTOF/MS) technique and a total of 54 compounds belonging to different groups were detected and identified. These findings help to uncover the relation of therapeutic activity of EBN. The EBN was further extracted with AcOEt and BuOH. The AcOEt extract was fractionated into three fractions (F₁−F₃), and the high triglyceride content in F₂ was verified by gC-FID. The three groups of fatty acids discovered in EBN are 48.43 % of poly-unsaturated (PUFA), 25.35 % of saturated fatty acids (SFA) and 24.74 % of mono-unsaturated fat (MUFA). This is the first time to report results ofEBN, BuOH, and AcOEt extracts and of fraction F₂ (TEBN) on their analysis for their antioxidant activities by DPPH, ABTS and catalase assay and for their paraoxonase and anti-tyrosinase activities. The results showed that TEBN exhibited the significant bioactivity in all assays. These findings suggest that TEBN is a good source for natural bioactive compounds in promoting body vigor. Current work widened the content of EBN especially on the triglyceride and also marked the content of specific location (Johor, Malaysia) of EBN origin.

Original language	English
Article number	e1900419
Number of pages	14
Journal	Chemistry and Biodiversity
Volume	17
Issue number	1
DOIs	https://doi.org/10.1002/cbdv.201900419
Publication status	Published - 1 Jan 2020
Externally published	Yes

Bibliographical note

Funding Information:
The authors are grateful to Universiti Teknologi Malaysia (UTM) via research grant (Grant No. R.J130000.7309.4B144) for the financial support of this study. The authors also thank the Faculty of Science of Monastir, University of Monastir, Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity, Team of Medicinal Chemistry and Natural Products, Tunisia, for providing the necessary research facilities and timely support in order to complete research work.

Funding

The authors are grateful to Universiti Teknologi Malaysia (UTM) via research grant (Grant No. R.J130000.7309.4B144) for the financial support of this study. The authors also thank the Faculty of Science of Monastir, University of Monastir, Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity, Team of Medicinal Chemistry and Natural Products, Tunisia, for providing the necessary research facilities and timely support in order to complete research work.

Keywords

characterization
house edible bird's nest
Malaysia
non-polar compounds
polar compounds
Nesting Behavior
Nutritive Value
Proteins/analysis
Birds
Chromatography, High Pressure Liquid
Food Analysis
Animals
Amino Acids/analysis
Fatty Acids/analysis
Biological Products/analysis

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10.1002/cbdv.201900419

Cite this

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title = "Characterization of Polar and Non-Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia",

abstract = "This work investigated the polar (PC: protein, amino acid and metabolite) and non-polar (NPC: fatty acid) compounds and bioactivity characteristics of the EBN harvested from the state of Johor in Malaysia. The electrophoretic gels exhibited 15 protein bands (16–173 kD) with unique protein profile. Amino acids analysis by AccQ⋅Tag method revealed 18 types of amino acids in EBN. Metabolite profiling was performed using High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometer (HPLC-QTOF/MS) technique and a total of 54 compounds belonging to different groups were detected and identified. These findings help to uncover the relation of therapeutic activity of EBN. The EBN was further extracted with AcOEt and BuOH. The AcOEt extract was fractionated into three fractions (F1−F3), and the high triglyceride content in F2 was verified by gC-FID. The three groups of fatty acids discovered in EBN are 48.43 % of poly-unsaturated (PUFA), 25.35 % of saturated fatty acids (SFA) and 24.74 % of mono-unsaturated fat (MUFA). This is the first time to report results ofEBN, BuOH, and AcOEt extracts and of fraction F2 (TEBN) on their analysis for their antioxidant activities by DPPH, ABTS and catalase assay and for their paraoxonase and anti-tyrosinase activities. The results showed that TEBN exhibited the significant bioactivity in all assays. These findings suggest that TEBN is a good source for natural bioactive compounds in promoting body vigor. Current work widened the content of EBN especially on the triglyceride and also marked the content of specific location (Johor, Malaysia) of EBN origin.",

keywords = "characterization, house edible bird's nest, Malaysia, non-polar compounds, polar compounds, Nesting Behavior, Nutritive Value, Proteins/analysis, Birds, Chromatography, High Pressure Liquid, Food Analysis, Animals, Amino Acids/analysis, Fatty Acids/analysis, Biological Products/analysis",

author = "Lee, {Ting Hun} and Lee, {Chia Hau} and Azmi, {Nurul Alia} and Supparmaniam Kavita and Syieluing Wong and Mansour Znati and {Ben Jannet}, Hichem",

note = "Funding Information: The authors are grateful to Universiti Teknologi Malaysia (UTM) via research grant (Grant No. R.J130000.7309.4B144) for the financial support of this study. The authors also thank the Faculty of Science of Monastir, University of Monastir, Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity, Team of Medicinal Chemistry and Natural Products, Tunisia, for providing the necessary research facilities and timely support in order to complete research work. ",

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doi = "10.1002/cbdv.201900419",

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AU - Lee, Ting Hun

AU - Lee, Chia Hau

AU - Azmi, Nurul Alia

AU - Kavita, Supparmaniam

AU - Wong, Syieluing

AU - Znati, Mansour

AU - Ben Jannet, Hichem

N1 - Funding Information: The authors are grateful to Universiti Teknologi Malaysia (UTM) via research grant (Grant No. R.J130000.7309.4B144) for the financial support of this study. The authors also thank the Faculty of Science of Monastir, University of Monastir, Laboratory of Heterocyclic Chemistry, Natural Products and Reactivity, Team of Medicinal Chemistry and Natural Products, Tunisia, for providing the necessary research facilities and timely support in order to complete research work.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - This work investigated the polar (PC: protein, amino acid and metabolite) and non-polar (NPC: fatty acid) compounds and bioactivity characteristics of the EBN harvested from the state of Johor in Malaysia. The electrophoretic gels exhibited 15 protein bands (16–173 kD) with unique protein profile. Amino acids analysis by AccQ⋅Tag method revealed 18 types of amino acids in EBN. Metabolite profiling was performed using High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometer (HPLC-QTOF/MS) technique and a total of 54 compounds belonging to different groups were detected and identified. These findings help to uncover the relation of therapeutic activity of EBN. The EBN was further extracted with AcOEt and BuOH. The AcOEt extract was fractionated into three fractions (F1−F3), and the high triglyceride content in F2 was verified by gC-FID. The three groups of fatty acids discovered in EBN are 48.43 % of poly-unsaturated (PUFA), 25.35 % of saturated fatty acids (SFA) and 24.74 % of mono-unsaturated fat (MUFA). This is the first time to report results ofEBN, BuOH, and AcOEt extracts and of fraction F2 (TEBN) on their analysis for their antioxidant activities by DPPH, ABTS and catalase assay and for their paraoxonase and anti-tyrosinase activities. The results showed that TEBN exhibited the significant bioactivity in all assays. These findings suggest that TEBN is a good source for natural bioactive compounds in promoting body vigor. Current work widened the content of EBN especially on the triglyceride and also marked the content of specific location (Johor, Malaysia) of EBN origin.

AB - This work investigated the polar (PC: protein, amino acid and metabolite) and non-polar (NPC: fatty acid) compounds and bioactivity characteristics of the EBN harvested from the state of Johor in Malaysia. The electrophoretic gels exhibited 15 protein bands (16–173 kD) with unique protein profile. Amino acids analysis by AccQ⋅Tag method revealed 18 types of amino acids in EBN. Metabolite profiling was performed using High-Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometer (HPLC-QTOF/MS) technique and a total of 54 compounds belonging to different groups were detected and identified. These findings help to uncover the relation of therapeutic activity of EBN. The EBN was further extracted with AcOEt and BuOH. The AcOEt extract was fractionated into three fractions (F1−F3), and the high triglyceride content in F2 was verified by gC-FID. The three groups of fatty acids discovered in EBN are 48.43 % of poly-unsaturated (PUFA), 25.35 % of saturated fatty acids (SFA) and 24.74 % of mono-unsaturated fat (MUFA). This is the first time to report results ofEBN, BuOH, and AcOEt extracts and of fraction F2 (TEBN) on their analysis for their antioxidant activities by DPPH, ABTS and catalase assay and for their paraoxonase and anti-tyrosinase activities. The results showed that TEBN exhibited the significant bioactivity in all assays. These findings suggest that TEBN is a good source for natural bioactive compounds in promoting body vigor. Current work widened the content of EBN especially on the triglyceride and also marked the content of specific location (Johor, Malaysia) of EBN origin.

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KW - Birds

KW - Chromatography, High Pressure Liquid

KW - Food Analysis

KW - Animals

KW - Amino Acids/analysis

KW - Fatty Acids/analysis

KW - Biological Products/analysis

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SN - 1612-1872

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Characterization of Polar and Non-Polar Compounds of House Edible Bird's Nest (EBN) from Johor, Malaysia

Abstract

Bibliographical note

Funding

Keywords

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