Cellular integration of an enzyme-loaded polymersome nanoreactor

S.F.M. van Dongen; W.P.R. Verdurmen; R.J.R.W. Peters; R.J.M. Nolte; R. Brock; J.C.M. van Hest

doi:10.1002/anie.201002655

Cellular integration of an enzyme-loaded polymersome nanoreactor

S.F.M. van Dongen, W.P.R. Verdurmen, R.J.R.W. Peters, R.J.M. Nolte, R. Brock, J.C.M. van Hest

ICMS Business Operations

Research output: Contribution to journal › Article › Academic › peer-review

122 Citations (Scopus)

Abstract

Cells with implants: Porous enzyme-loaded polymersomes were constructed that display the cell-penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. Polymersomes with horseradish peroxidase as a model cargo enzyme displayed sustained intracellular activity.

Original language	English
Pages (from-to)	7213-7216
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	49
Issue number	40
DOIs	https://doi.org/10.1002/anie.201002655
Publication status	Published - 24 Sept 2010

Keywords

Bioorganic chemistry
Cell-penetrating peptides
Nanotechnology
Polymersomes

Access to Document

10.1002/anie.201002655

Cite this

@article{15784ee40658431d90905830448c7bfe,

title = "Cellular integration of an enzyme-loaded polymersome nanoreactor",

abstract = "Cells with implants: Porous enzyme-loaded polymersomes were constructed that display the cell-penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. Polymersomes with horseradish peroxidase as a model cargo enzyme displayed sustained intracellular activity.",

keywords = "Bioorganic chemistry, Cell-penetrating peptides, Nanotechnology, Polymersomes",

author = "{van Dongen}, S.F.M. and W.P.R. Verdurmen and R.J.R.W. Peters and R.J.M. Nolte and R. Brock and {van Hest}, J.C.M.",

year = "2010",

month = sep,

day = "24",

doi = "10.1002/anie.201002655",

language = "English",

volume = "49",

pages = "7213--7216",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "Wiley",

number = "40",

}

TY - JOUR

T1 - Cellular integration of an enzyme-loaded polymersome nanoreactor

AU - van Dongen, S.F.M.

AU - Verdurmen, W.P.R.

AU - Peters, R.J.R.W.

AU - Nolte, R.J.M.

AU - Brock, R.

AU - van Hest, J.C.M.

PY - 2010/9/24

Y1 - 2010/9/24

N2 - Cells with implants: Porous enzyme-loaded polymersomes were constructed that display the cell-penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. Polymersomes with horseradish peroxidase as a model cargo enzyme displayed sustained intracellular activity.

AB - Cells with implants: Porous enzyme-loaded polymersomes were constructed that display the cell-penetrating peptide tat on their surfaces. These nanoreactors are taken up by mammalian cells through macropinocytosis. Inside the cells, the polymersomes are only partially routed to acidic compartments. Polymersomes with horseradish peroxidase as a model cargo enzyme displayed sustained intracellular activity.

KW - Bioorganic chemistry

KW - Cell-penetrating peptides

KW - Nanotechnology

KW - Polymersomes

UR - http://www.scopus.com/inward/record.url?scp=77957369621&partnerID=8YFLogxK

U2 - 10.1002/anie.201002655

DO - 10.1002/anie.201002655

M3 - Article

C2 - 20737527

AN - SCOPUS:77957369621

SN - 1433-7851

VL - 49

SP - 7213

EP - 7216

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 40

ER -

Cellular integration of an enzyme-loaded polymersome nanoreactor

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this