Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton

Aysegul Dede Eren; Amy W.A. Lucassen; Urandelger Tuvshindorj; Roman Truckenmüller; Stefan Giselbrecht; E. Deniz Eren; Mehmet Orhan Tas; Phanikrishna Sudarsanam; Jan de Boer

doi:10.3389/fcell.2022.863721

Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton

Aysegul Dede Eren, Amy W.A. Lucassen, Urandelger Tuvshindorj, Roman Truckenmüller, Stefan Giselbrecht, E. Deniz Eren, Mehmet Orhan Tas, Phanikrishna Sudarsanam, Jan de Boer (Corresponding author)

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Abstract

Cells probe their environment and adapt their shape accordingly via the organization of focal adhesions and the actin cytoskeleton. In an earlier publication, we described the relationship between cell shape and physiology, for example, shape-induced differentiation, metabolism, and proliferation in mesenchymal stem cells and tenocytes. In this study, we investigated how these cells organize their adhesive machinery over time when exposed to microfabricated surfaces of different topographies and adhesive island geometries. We further examined the reciprocal interaction between stress fiber and focal adhesion formation by pharmacological perturbations. Our results confirm the current literature that spatial organization of adhesive sites determines the ability to form focal adhesions and stress fibers. Therefore, cells on roughened surfaces have smaller focal adhesion and fewer stress fibers. Our results further highlight the importance of integrin-mediated adhesion in the adaptive properties of cells and provide clear links to the development of bioactive materials.

Original language	English
Article number	863721
Number of pages	16
Journal	Frontiers in Cell and Developmental Biology
Volume	10
DOIs	https://doi.org/10.3389/fcell.2022.863721
Publication status	Published - 3 Jun 2022

Bibliographical note

Funding Information:
ADE and JdB acknowledge the funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 676338. UT, SG, and RT acknowledge financial support by the Dutch province of Limburg (program “Limburg INvesteert in haar Kenniseconomie/LINK”; nos. SAS-2014-00837 and SAS-2018-02477), and SG and RT by the Gravitation Program (project ‘Materials-driven regeneration; no. 024.003.013) of the Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijk Onderzoek; NWO).

Funding

ADE and JdB acknowledge the funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 676338. UT, SG, and RT acknowledge financial support by the Dutch province of Limburg (program “Limburg INvesteert in haar Kenniseconomie/LINK”; nos. SAS-2014-00837 and SAS-2018-02477), and SG and RT by the Gravitation Program (project ‘Materials-driven regeneration; no. 024.003.013) of the Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijk Onderzoek; NWO).

Keywords

cell shape
focal adhesion
mechanotransduction
stress fibers
tenocytes

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10.3389/fcell.2022.863721

fcell-10-863721Final published version, 4.6 MB

Cite this

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title = "Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton",

abstract = "Cells probe their environment and adapt their shape accordingly via the organization of focal adhesions and the actin cytoskeleton. In an earlier publication, we described the relationship between cell shape and physiology, for example, shape-induced differentiation, metabolism, and proliferation in mesenchymal stem cells and tenocytes. In this study, we investigated how these cells organize their adhesive machinery over time when exposed to microfabricated surfaces of different topographies and adhesive island geometries. We further examined the reciprocal interaction between stress fiber and focal adhesion formation by pharmacological perturbations. Our results confirm the current literature that spatial organization of adhesive sites determines the ability to form focal adhesions and stress fibers. Therefore, cells on roughened surfaces have smaller focal adhesion and fewer stress fibers. Our results further highlight the importance of integrin-mediated adhesion in the adaptive properties of cells and provide clear links to the development of bioactive materials.",

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author = "\{Dede Eren\}, Aysegul and Lucassen, \{Amy W.A.\} and Urandelger Tuvshindorj and Roman Truckenm{\"u}ller and Stefan Giselbrecht and Eren, \{E. Deniz\} and Tas, \{Mehmet Orhan\} and Phanikrishna Sudarsanam and \{de Boer\}, Jan",

note = "Funding Information: ADE and JdB acknowledge the funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 676338. UT, SG, and RT acknowledge financial support by the Dutch province of Limburg (program “Limburg INvesteert in haar Kenniseconomie/LINK”; nos. SAS-2014-00837 and SAS-2018-02477), and SG and RT by the Gravitation Program (project {\textquoteleft}Materials-driven regeneration; no. 024.003.013) of the Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijk Onderzoek; NWO). ",

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journal = "Frontiers in Cell and Developmental Biology",

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AU - Dede Eren, Aysegul

AU - Lucassen, Amy W.A.

AU - Tuvshindorj, Urandelger

AU - Truckenmüller, Roman

AU - Giselbrecht, Stefan

AU - Eren, E. Deniz

AU - Tas, Mehmet Orhan

AU - Sudarsanam, Phanikrishna

AU - de Boer, Jan

N1 - Funding Information: ADE and JdB acknowledge the funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No. 676338. UT, SG, and RT acknowledge financial support by the Dutch province of Limburg (program “Limburg INvesteert in haar Kenniseconomie/LINK”; nos. SAS-2014-00837 and SAS-2018-02477), and SG and RT by the Gravitation Program (project ‘Materials-driven regeneration; no. 024.003.013) of the Dutch Research Council (Nederlandse Organisatie voor Wetenschappelijk Onderzoek; NWO).

PY - 2022/6/3

Y1 - 2022/6/3

N2 - Cells probe their environment and adapt their shape accordingly via the organization of focal adhesions and the actin cytoskeleton. In an earlier publication, we described the relationship between cell shape and physiology, for example, shape-induced differentiation, metabolism, and proliferation in mesenchymal stem cells and tenocytes. In this study, we investigated how these cells organize their adhesive machinery over time when exposed to microfabricated surfaces of different topographies and adhesive island geometries. We further examined the reciprocal interaction between stress fiber and focal adhesion formation by pharmacological perturbations. Our results confirm the current literature that spatial organization of adhesive sites determines the ability to form focal adhesions and stress fibers. Therefore, cells on roughened surfaces have smaller focal adhesion and fewer stress fibers. Our results further highlight the importance of integrin-mediated adhesion in the adaptive properties of cells and provide clear links to the development of bioactive materials.

AB - Cells probe their environment and adapt their shape accordingly via the organization of focal adhesions and the actin cytoskeleton. In an earlier publication, we described the relationship between cell shape and physiology, for example, shape-induced differentiation, metabolism, and proliferation in mesenchymal stem cells and tenocytes. In this study, we investigated how these cells organize their adhesive machinery over time when exposed to microfabricated surfaces of different topographies and adhesive island geometries. We further examined the reciprocal interaction between stress fiber and focal adhesion formation by pharmacological perturbations. Our results confirm the current literature that spatial organization of adhesive sites determines the ability to form focal adhesions and stress fibers. Therefore, cells on roughened surfaces have smaller focal adhesion and fewer stress fibers. Our results further highlight the importance of integrin-mediated adhesion in the adaptive properties of cells and provide clear links to the development of bioactive materials.

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KW - mechanotransduction

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KW - tenocytes

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Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton

Abstract

Bibliographical note

Funding

Keywords

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Center for Multiscale Electron Microscopy (CMEM)

Cite this

Cells Dynamically Adapt to Surface Geometry by Remodeling Their Focal Adhesions and Actin Cytoskeleton

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

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Center for Multiscale Electron Microscopy (CMEM)

Cite this