Cell Sources for Human In vitro Bone Models

Sana Ansari, Keita Ito, Sandra Hofmann (Corresponding author)

Research output: Contribution to journalReview articlepeer-review

Abstract

PURPOSE OF REVIEW: One aim in bone tissue engineering is to develop human cell-based, 3D in vitro bone models to study bone physiology and pathology. Due to the heterogeneity of cells among patients, patient's own cells are needed to be obtained, ideally, from one single cell source. This review attempts to identify the appropriate cell sources for development of such models.

RECENT FINDINGS: Bone marrow and peripheral blood are considered as suitable sources for extraction of osteoblast/osteocyte and osteoclast progenitor cells. Recent studies on these cell sources have shown no significant differences between isolated progenitor cells. However, various parameters such as medium composition affect the cell's proliferation and differentiation potential which could make the peripheral blood-derived stem cells superior to the ones from bone marrow. Peripheral blood can be considered a suitable source for osteoblast/osteocyte and osteoclast progenitor cells, being less invasive for the patient. However, more investigations are needed focusing on extraction and differentiation of both cell types from the same donor sample of peripheral blood.

Original languageEnglish
Pages (from-to)88-100
Number of pages13
JournalCurrent Osteoporosis Reports
Volume19
Issue number1
Early online date15 Jan 2021
DOIs
Publication statusPublished - 15 Jan 2021

Keywords

  • Bone marrow
  • Hematopoietic stem cell
  • In vitro bone model
  • Mesenchymal stem cell
  • Peripheral blood
  • Personalized medicine

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