Cell-perceived substrate curvature dynamically coordinates the direction, speed, and persistence of stromal cell migration

Maike Werner, Ansgar Petersen, Nicholas Kurniawan (Corresponding author), Carlijn Bouten

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
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Abstract

Adherent cells residing within tissues or biomaterials are presented with 3D geometrical cues from their environment, often in the form of local surface curvatures. While there is growing evidence that cellular decision‐making is influenced by substrate curvature, the effect of physiologically relevant, cell‐scale anisotropic curvatures remains poorly understood. This study systematically explores the migration behavior of human bone marrow stromal cells (hBMSCs) on a library of anisotropic curved structures. Analysis of cell trajectories reveals that, on convex cylindrical structures, hBMSC migration speed and persistence are strongly governed by the cellular orientation on the curved structure, while migration on concave cylindrical structures is characterized by fast but non‐aligned and non‐persistent migration. Concurrent presentation of concave and convex substrates on toroidal structures induces migration in the direction where hBMSCs can most effectively avoid cell bending. These distinct migration behaviors are found to be universally explained by the cell‐perceived substrate curvature, which on anisotropic curved structures is dependent on both the temporally varying cell orientation and the 3D cellular morphology. This work demonstrates that cell migration is dynamically guided by the perceived curvature of the underlying substrate, providing an important biomaterial design parameter for instructing cell migration in tissue engineering and regenerative medicine.
Original languageEnglish
Article number1900080
Number of pages11
JournalAdvanced Biosystems
Volume3
Issue number10
DOIs
Publication statusPublished - 5 Sep 2019

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Stromal Cells
Cell Movement
Mesenchymal Stromal Cells
Bone
Biocompatible Materials
Substrates
Biomaterials
Regenerative Medicine
Tissue Engineering
Tissue engineering
Libraries
Cues
Trajectories
Tissue
Direction compound

Keywords

  • cell adhesion
  • cell migration
  • persistence
  • substrate curvature
  • tissue geometry

Cite this

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title = "Cell-perceived substrate curvature dynamically coordinates the direction, speed, and persistence of stromal cell migration",
abstract = "Adherent cells residing within tissues or biomaterials are presented with 3D geometrical cues from their environment, often in the form of local surface curvatures. While there is growing evidence that cellular decision‐making is influenced by substrate curvature, the effect of physiologically relevant, cell‐scale anisotropic curvatures remains poorly understood. This study systematically explores the migration behavior of human bone marrow stromal cells (hBMSCs) on a library of anisotropic curved structures. Analysis of cell trajectories reveals that, on convex cylindrical structures, hBMSC migration speed and persistence are strongly governed by the cellular orientation on the curved structure, while migration on concave cylindrical structures is characterized by fast but non‐aligned and non‐persistent migration. Concurrent presentation of concave and convex substrates on toroidal structures induces migration in the direction where hBMSCs can most effectively avoid cell bending. These distinct migration behaviors are found to be universally explained by the cell‐perceived substrate curvature, which on anisotropic curved structures is dependent on both the temporally varying cell orientation and the 3D cellular morphology. This work demonstrates that cell migration is dynamically guided by the perceived curvature of the underlying substrate, providing an important biomaterial design parameter for instructing cell migration in tissue engineering and regenerative medicine.",
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Cell-perceived substrate curvature dynamically coordinates the direction, speed, and persistence of stromal cell migration. / Werner, Maike; Petersen, Ansgar; Kurniawan, Nicholas (Corresponding author); Bouten, Carlijn.

In: Advanced Biosystems, Vol. 3, No. 10, 1900080, 05.09.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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