TY - JOUR
T1 - Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks
AU - Dubuc, Emilien
AU - Pieters, Pascal A.
AU - van der Linden, Ardjan J.
AU - van Hest, Jan C.M.
AU - Huck, Wilhelm T.S.
AU - de Greef, Tom F.A.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Recent efforts in synthetic biology have shown the possibility of engineering distributed functions in populations of living cells, which requires the development of highly orthogonal, genetically encoded communication pathways. Cell-free transcription-translation (TXTL) reactions encapsulated in microcompartments enable prototyping of molecular communication channels and their integration into engineered genetic circuits by mimicking critical cell features, such as gene expression, cell size, and cell individuality within a community. In this review, we discuss the uses of cell-free transcription–translation reactions for the development of synthetic genetic circuits, with a special focus on the use of microcompartments supporting this reaction. We highlight several studies where molecular communication between non-living microcompartments and living cells have been successfully engineered.
AB - Recent efforts in synthetic biology have shown the possibility of engineering distributed functions in populations of living cells, which requires the development of highly orthogonal, genetically encoded communication pathways. Cell-free transcription-translation (TXTL) reactions encapsulated in microcompartments enable prototyping of molecular communication channels and their integration into engineered genetic circuits by mimicking critical cell features, such as gene expression, cell size, and cell individuality within a community. In this review, we discuss the uses of cell-free transcription–translation reactions for the development of synthetic genetic circuits, with a special focus on the use of microcompartments supporting this reaction. We highlight several studies where molecular communication between non-living microcompartments and living cells have been successfully engineered.
UR - http://www.scopus.com/inward/record.url?scp=85059021795&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2018.10.006
DO - 10.1016/j.copbio.2018.10.006
M3 - Review article
C2 - 30594098
AN - SCOPUS:85059021795
VL - 58
SP - 72
EP - 80
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
SN - 0958-1669
ER -