Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

Guansheng Du; Jaap M.J. den Toonder; Qun Fang

Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

Guansheng Du
, Jaap M.J. den Toonder
, Qun Fang

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

Original language	English
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Place of Publication	Washington
Publisher	Royal Society of Chemistry
Pages	965-967
Number of pages	3
ISBN (Print)	9780979806452
Publication status	Published - 1 Nov 2012
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 Conference number: 16

Conference

Conference	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Abbreviated title	MicroTAS 2012
Country/Territory	Japan
City	Okinawa
Period	28/10/12 → 1/11/12

Keywords

Cell
Drug combination
High-throughput
Microfluidic droplet

Cite this

@inproceedings{a024171165f14f2b84d33e484274b372,

title = "Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system",

abstract = "We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.",

keywords = "Cell, Drug combination, High-throughput, Microfluidic droplet",

author = "Guansheng Du and \{den Toonder\}, \{Jaap M.J.\} and Qun Fang",

year = "2012",

month = nov,

day = "1",

language = "English",

isbn = "9780979806452",

pages = "965--967",

booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

publisher = "Royal Society of Chemistry",

address = "United Kingdom",

note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",

}

Du, G, den Toonder, JMJ & Fang, Q 2012, Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Royal Society of Chemistry, Washington, pp. 965-967, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 28/10/12.

Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system. / Du, Guansheng; den Toonder, Jaap M.J.; Fang, Qun.
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Washington: Royal Society of Chemistry, 2012. p. 965-967.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

AU - Du, Guansheng

AU - den Toonder, Jaap M.J.

AU - Fang, Qun

N1 - Conference code: 16

PY - 2012/11/1

Y1 - 2012/11/1

N2 - We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

AB - We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

KW - Cell

KW - Drug combination

KW - High-throughput

KW - Microfluidic droplet

UR - https://www.scopus.com/pages/publications/84901776498

M3 - Conference contribution

AN - SCOPUS:84901776498

SN - 9780979806452

SP - 965

EP - 967

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - Royal Society of Chemistry

CY - Washington

T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Y2 - 28 October 2012 through 1 November 2012

ER -

Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

Abstract

Conference

Keywords

Other files and links

Fingerprint

Cite this