Cell-based schedule dependent drug combination screening with a droplet-based microfluidic system

Guansheng Du, Jaap M.J. den Toonder, Qun Fang

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Place of PublicationWashington
PublisherRoyal Society of Chemistry
Pages965-967
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 1 Nov 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: 28 Oct 20121 Nov 2012
Conference number: 16

Conference

Conference16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Abbreviated titleMicroTAS 2012
Country/TerritoryJapan
CityOkinawa
Period28/10/121/11/12

Keywords

  • Cell
  • Drug combination
  • High-throughput
  • Microfluidic droplet

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