Abstract
We proposed an integrated and robust microfluidic platform to perform cell-based schedule dependent drug combination screening. Multiple manipulations of cells in droplets, including cell dispensing, culture, drug stimulation and viability test were performed based on a 2D microfluidic droplet array. The screening of flavopiridol (Fla) and 5-fluorouracil (5Flu) in different sequence was carried out in 342 addressable droplets, with a consumption of ∼3 μg drug for each experiment.
Original language | English |
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Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
Place of Publication | Washington |
Publisher | Royal Society of Chemistry |
Pages | 965-967 |
Number of pages | 3 |
ISBN (Print) | 9780979806452 |
Publication status | Published - 1 Nov 2012 |
Event | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: 28 Oct 2012 → 1 Nov 2012 Conference number: 16 |
Conference
Conference | 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
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Abbreviated title | MicroTAS 2012 |
Country/Territory | Japan |
City | Okinawa |
Period | 28/10/12 → 1/11/12 |
Keywords
- Cell
- Drug combination
- High-throughput
- Microfluidic droplet