Catenane versus ring: do both assemblies of CS2 hydrolase exhibit the same stability and catalytic activity?

M.B. van Eldijk, B.J. Pieters, V.A. Mikhailov, C.V. Robinson, J.C.M. van Hest, J. Mecinović

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)
15 Downloads (Pure)

Abstract

Catenane structures, in which two or more rings are mechanically interlocked, have historically occupied one of the central places in the field of supramolecular chemistry. In contrast to synthetic small-molecule catenanes, examples of naturally-occurring catenanes are scarce. Here, we report thermodynamic and enzymatic studies on CS2 hydrolase, which exists in solution as a mixture of unique hexadecameric catenane and octameric ring forms. A combination of field-flow fractionation coupled to multi-angle laser light scattering (FFF-MALLS) and native mass spectrometric analyses revealed that the catenane form is converted into the ring form at elevated temperatures, whereas the ring does not assemble into the catenane under the same conditions. Measurements of the enzyme kinetics for the conversion of CS2 into COS and H2S showed that the ring form of CS2 hydrolase possesses higher enzyme efficiency (per monomer) than the catenane form, whereas the catenane form is overall more active (per assembly).

Original languageEnglish
Pages (from-to)2879-2884
Number of pages6
JournalChemical Science
Volume5
Issue number7
DOIs
Publication statusPublished - 2014
Externally publishedYes

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