Abstract
Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments. Mimicking cells: Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanylaminoethyl) thiophene) (PS-b-PIAT) nanoreactors have been encapsulated together with free enzymes and substrates in a larger polymersome to form a multicompartmentalized structure, which shows structural resemblance to a cell and its organelles (see picture). An original cofactor-dependent three-enzyme cascade reaction, with either compatible or incompatible enzymes, takes place across multiple compartments.
Original language | English |
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Pages (from-to) | 146-150 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 53 |
Issue number | 1 |
DOIs | |
Publication status | Published - 3 Jan 2014 |
Externally published | Yes |
Keywords
- artificial cells
- cascade reactions
- enzyme catalysis
- nanoreactors
- polymersomes