Cascade reactions in multicompartmentalized polymersomes

R.J.R.W. Peters, M. Marguet, S. Marais, M.W. Fraaije, J.C.M. van Hest, S. Lecommandoux

Research output: Contribution to journalArticleAcademicpeer-review

307 Citations (Scopus)
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Abstract

Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanyl-aminoethyl)thiophene) (PS-b-PIAT) nanoreactors are encapsulated together with free enzymes and substrates in a larger polybutadiene-b-poly(ethylene oxide) (PB-b-PEO) polymersome, forming a multicompartmentalized structure, which shows structural resemblance to the cell and its organelles. An original cofactor-dependent three-enzyme cascade reaction is performed, using either compatible or incompatible enzymes, which takes place across multiple compartments. Mimicking cells: Enzyme-filled polystyrene-b-poly(3-(isocyano-L-alanylaminoethyl) thiophene) (PS-b-PIAT) nanoreactors have been encapsulated together with free enzymes and substrates in a larger polymersome to form a multicompartmentalized structure, which shows structural resemblance to a cell and its organelles (see picture). An original cofactor-dependent three-enzyme cascade reaction, with either compatible or incompatible enzymes, takes place across multiple compartments.

Original languageEnglish
Pages (from-to)146-150
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number1
DOIs
Publication statusPublished - 3 Jan 2014
Externally publishedYes

Keywords

  • artificial cells
  • cascade reactions
  • enzyme catalysis
  • nanoreactors
  • polymersomes

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