Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells

S. Hofmann, S. Knecht, R. Langer, D.L. Kaplan, G. Vunjak-Novakovic, H.P. Merkle, L. Meinel

Research output: Contribution to journalArticleAcademicpeer-review

150 Citations (Scopus)
1 Downloads (Pure)

Abstract

Silk fibroin scaffolds were studied as a new biomaterial option for tissue-engineered cartilage-like tissue. Human bone marrow-derived mesenchymal stem cells (MSCs) were seeded on silk, collagen, and crosslinked collagen scaffolds and cultured for 21 days in serum-free chondrogenic medium. Cells proliferated more rapidly on the silk fibroin scaffolds than on the collagen matrices. The total content of glycosaminoglycan deposition was three times higher on silk as compared to collagen scaffolds. Glycosaminoglycan deposition coincided with overexpression of collagen type II and aggrecan genes. Cartilage-like tissue was homogeneously distributed throughout the entire silk scaffolds, while on the collagen and crosslinked collagen systems tissue formation was restricted to the outer rim, leaving a doughnut appearance. Round or angular-shaped cells resided in deep lacunae in the silk systems and stained positively for collagen type II. The aggregate modulus of the tissue-engineered cartilage constructs was more than 2-fold higher than that of the unseeded silk scaffold controls. These results suggest that silk fibroin scaffolds are suitable biomaterial substrates for autologous cartilage tissue engineering in serum-free medium and enable mechanical improvements along with compositional features suitable for durable implants to generate or regenerate cartilage. © Mary Ann Liebert, Inc.
Original languageEnglish
Pages (from-to)2729-2738
Number of pages10
JournalTissue Engineering
Volume12
Issue number10
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Silk
Tissue Engineering
Mesenchymal Stromal Cells
Cartilage
Collagen
Fibroins
Collagen Type II
Serum-Free Culture Media
Biocompatible Materials
Glycosaminoglycans
Aggrecans
Bone Marrow

Cite this

Hofmann, S., Knecht, S., Langer, R., Kaplan, D. L., Vunjak-Novakovic, G., Merkle, H. P., & Meinel, L. (2006). Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells. Tissue Engineering, 12(10), 2729-2738. https://doi.org/10.1089/ten.2006.12.2729
Hofmann, S. ; Knecht, S. ; Langer, R. ; Kaplan, D.L. ; Vunjak-Novakovic, G. ; Merkle, H.P. ; Meinel, L. / Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells. In: Tissue Engineering. 2006 ; Vol. 12, No. 10. pp. 2729-2738.
@article{372c8340649b4aad9f0bd3d77fe96225,
title = "Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells",
abstract = "Silk fibroin scaffolds were studied as a new biomaterial option for tissue-engineered cartilage-like tissue. Human bone marrow-derived mesenchymal stem cells (MSCs) were seeded on silk, collagen, and crosslinked collagen scaffolds and cultured for 21 days in serum-free chondrogenic medium. Cells proliferated more rapidly on the silk fibroin scaffolds than on the collagen matrices. The total content of glycosaminoglycan deposition was three times higher on silk as compared to collagen scaffolds. Glycosaminoglycan deposition coincided with overexpression of collagen type II and aggrecan genes. Cartilage-like tissue was homogeneously distributed throughout the entire silk scaffolds, while on the collagen and crosslinked collagen systems tissue formation was restricted to the outer rim, leaving a doughnut appearance. Round or angular-shaped cells resided in deep lacunae in the silk systems and stained positively for collagen type II. The aggregate modulus of the tissue-engineered cartilage constructs was more than 2-fold higher than that of the unseeded silk scaffold controls. These results suggest that silk fibroin scaffolds are suitable biomaterial substrates for autologous cartilage tissue engineering in serum-free medium and enable mechanical improvements along with compositional features suitable for durable implants to generate or regenerate cartilage. {\circledC} Mary Ann Liebert, Inc.",
author = "S. Hofmann and S. Knecht and R. Langer and D.L. Kaplan and G. Vunjak-Novakovic and H.P. Merkle and L. Meinel",
year = "2006",
doi = "10.1089/ten.2006.12.2729",
language = "English",
volume = "12",
pages = "2729--2738",
journal = "Tissue Engineering",
issn = "1076-3279",
publisher = "Mary Ann Liebert Inc.",
number = "10",

}

Hofmann, S, Knecht, S, Langer, R, Kaplan, DL, Vunjak-Novakovic, G, Merkle, HP & Meinel, L 2006, 'Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells', Tissue Engineering, vol. 12, no. 10, pp. 2729-2738. https://doi.org/10.1089/ten.2006.12.2729

Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells. / Hofmann, S.; Knecht, S.; Langer, R.; Kaplan, D.L.; Vunjak-Novakovic, G.; Merkle, H.P.; Meinel, L.

In: Tissue Engineering, Vol. 12, No. 10, 2006, p. 2729-2738.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells

AU - Hofmann, S.

AU - Knecht, S.

AU - Langer, R.

AU - Kaplan, D.L.

AU - Vunjak-Novakovic, G.

AU - Merkle, H.P.

AU - Meinel, L.

PY - 2006

Y1 - 2006

N2 - Silk fibroin scaffolds were studied as a new biomaterial option for tissue-engineered cartilage-like tissue. Human bone marrow-derived mesenchymal stem cells (MSCs) were seeded on silk, collagen, and crosslinked collagen scaffolds and cultured for 21 days in serum-free chondrogenic medium. Cells proliferated more rapidly on the silk fibroin scaffolds than on the collagen matrices. The total content of glycosaminoglycan deposition was three times higher on silk as compared to collagen scaffolds. Glycosaminoglycan deposition coincided with overexpression of collagen type II and aggrecan genes. Cartilage-like tissue was homogeneously distributed throughout the entire silk scaffolds, while on the collagen and crosslinked collagen systems tissue formation was restricted to the outer rim, leaving a doughnut appearance. Round or angular-shaped cells resided in deep lacunae in the silk systems and stained positively for collagen type II. The aggregate modulus of the tissue-engineered cartilage constructs was more than 2-fold higher than that of the unseeded silk scaffold controls. These results suggest that silk fibroin scaffolds are suitable biomaterial substrates for autologous cartilage tissue engineering in serum-free medium and enable mechanical improvements along with compositional features suitable for durable implants to generate or regenerate cartilage. © Mary Ann Liebert, Inc.

AB - Silk fibroin scaffolds were studied as a new biomaterial option for tissue-engineered cartilage-like tissue. Human bone marrow-derived mesenchymal stem cells (MSCs) were seeded on silk, collagen, and crosslinked collagen scaffolds and cultured for 21 days in serum-free chondrogenic medium. Cells proliferated more rapidly on the silk fibroin scaffolds than on the collagen matrices. The total content of glycosaminoglycan deposition was three times higher on silk as compared to collagen scaffolds. Glycosaminoglycan deposition coincided with overexpression of collagen type II and aggrecan genes. Cartilage-like tissue was homogeneously distributed throughout the entire silk scaffolds, while on the collagen and crosslinked collagen systems tissue formation was restricted to the outer rim, leaving a doughnut appearance. Round or angular-shaped cells resided in deep lacunae in the silk systems and stained positively for collagen type II. The aggregate modulus of the tissue-engineered cartilage constructs was more than 2-fold higher than that of the unseeded silk scaffold controls. These results suggest that silk fibroin scaffolds are suitable biomaterial substrates for autologous cartilage tissue engineering in serum-free medium and enable mechanical improvements along with compositional features suitable for durable implants to generate or regenerate cartilage. © Mary Ann Liebert, Inc.

U2 - 10.1089/ten.2006.12.2729

DO - 10.1089/ten.2006.12.2729

M3 - Article

C2 - 17518642

VL - 12

SP - 2729

EP - 2738

JO - Tissue Engineering

JF - Tissue Engineering

SN - 1076-3279

IS - 10

ER -