Capillary gas chromatography in cancer research

E.A. de Bruijn; P.A. Leclercq; A.T. Oosterom, van; U.R. Tjaden; C.J.H. van de Velde; L.M. Brauw, de; T.B.L.W. Marinelli, von

Capillary gas chromatography in cancer research

E.A. de Bruijn, P.A. Leclercq, A.T. Oosterom, van, U.R. Tjaden, C.J.H. van de Velde, L.M. Brauw, de, T.B.L.W. Marinelli, von

Chemical Engineering and Chemistry

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

Abstract

Monitoring of endogenous compounds and xenobiotics including anticancer drugs is of extreme importance in cancer research. The intrOduction of capillary columns allowed selective and sensitive monitoring of anticancer agents in body fluids of cancer patients such as cyclophosphamide and 5-fluorouracil. The use of short capillary columns even allows determination of underivatized drugs; then special care has to be taken for instability of compounds and selectivity towards metabolites. In running a capillary GC assay routinely for a metabolite of 5-fluorouracil, i.e. 5,6 dihydro-5-fluorouracil, instability In blood plasma could be demonstrated clearly. The data of the study showed that the instability can be overcome by proper sample pretreatment procedures. Adjusting the biological samples to pH3.5 and treatment of samples at T=40C Inhibit degradation of 5,6 dihydro-5-fluorouracil.

Original language	English
Title of host publication	Eighth International Symposium on Capillary Chromatography : Riva del Garda, Italy, May 19-21, 1987.
Editors	P. Sandra
Place of Publication	Heidelberg
Publisher	Huethig
Pages	754-763
Volume	2
Publication status	Published - 1987
Event	8th International Symposium on Capillary Chromatography, 1987 - Palazzo dei Congressi, Riva del Garda, Italy Duration: 19 May 1987 → 21 May 1987

Conference

Conference	8th International Symposium on Capillary Chromatography, 1987
Country/Territory	Italy
City	Riva del Garda
Period	19/05/87 → 21/05/87

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@inproceedings{5710daf66cc54faa94dcae4cf73e5b79,

title = "Capillary gas chromatography in cancer research",

abstract = "Monitoring of endogenous compounds and xenobiotics including anticancer drugs is of extreme importance in cancer research. The intrOduction of capillary columns allowed selective and sensitive monitoring of anticancer agents in body fluids of cancer patients such as cyclophosphamide and 5-fluorouracil. The use of short capillary columns even allows determination of underivatized drugs; then special care has to be taken for instability of compounds and selectivity towards metabolites. In running a capillary GC assay routinely for a metabolite of 5-fluorouracil, i.e. 5,6 dihydro-5-fluorouracil, instability In blood plasma could be demonstrated clearly. The data of the study showed that the instability can be overcome by proper sample pretreatment procedures. Adjusting the biological samples to pH3.5 and treatment of samples at T=40C Inhibit degradation of 5,6 dihydro-5-fluorouracil.",

author = "{de Bruijn}, E.A. and P.A. Leclercq and {Oosterom, van}, A.T. and U.R. Tjaden and {van de Velde}, C.J.H. and {Brauw, de}, L.M. and {Marinelli, von}, T.B.L.W.",

year = "1987",

language = "English",

volume = "2",

pages = "754--763",

editor = "P. Sandra",

booktitle = "Eighth International Symposium on Capillary Chromatography : Riva del Garda, Italy, May 19-21, 1987.",

publisher = "Huethig",

note = "8th International Symposium on Capillary Chromatography, 1987 ; Conference date: 19-05-1987 Through 21-05-1987",

}

de Bruijn, EA, Leclercq, PA, Oosterom, van, AT, Tjaden, UR, van de Velde, CJH, Brauw, de, LM & Marinelli, von, TBLW 1987, Capillary gas chromatography in cancer research. in P Sandra (ed.), Eighth International Symposium on Capillary Chromatography : Riva del Garda, Italy, May 19-21, 1987.. vol. 2, Huethig, Heidelberg, pp. 754-763, 8th International Symposium on Capillary Chromatography, 1987, Riva del Garda, Italy, 19/05/87.

Capillary gas chromatography in cancer research. / de Bruijn, E.A.; Leclercq, P.A.; Oosterom, van, A.T. et al.
Eighth International Symposium on Capillary Chromatography : Riva del Garda, Italy, May 19-21, 1987.. ed. / P. Sandra. Vol. 2 Heidelberg: Huethig, 1987. p. 754-763.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic

TY - GEN

T1 - Capillary gas chromatography in cancer research

AU - de Bruijn, E.A.

AU - Leclercq, P.A.

AU - Oosterom, van, A.T.

AU - Tjaden, U.R.

AU - van de Velde, C.J.H.

AU - Brauw, de, L.M.

AU - Marinelli, von, T.B.L.W.

PY - 1987

Y1 - 1987

N2 - Monitoring of endogenous compounds and xenobiotics including anticancer drugs is of extreme importance in cancer research. The intrOduction of capillary columns allowed selective and sensitive monitoring of anticancer agents in body fluids of cancer patients such as cyclophosphamide and 5-fluorouracil. The use of short capillary columns even allows determination of underivatized drugs; then special care has to be taken for instability of compounds and selectivity towards metabolites. In running a capillary GC assay routinely for a metabolite of 5-fluorouracil, i.e. 5,6 dihydro-5-fluorouracil, instability In blood plasma could be demonstrated clearly. The data of the study showed that the instability can be overcome by proper sample pretreatment procedures. Adjusting the biological samples to pH3.5 and treatment of samples at T=40C Inhibit degradation of 5,6 dihydro-5-fluorouracil.

AB - Monitoring of endogenous compounds and xenobiotics including anticancer drugs is of extreme importance in cancer research. The intrOduction of capillary columns allowed selective and sensitive monitoring of anticancer agents in body fluids of cancer patients such as cyclophosphamide and 5-fluorouracil. The use of short capillary columns even allows determination of underivatized drugs; then special care has to be taken for instability of compounds and selectivity towards metabolites. In running a capillary GC assay routinely for a metabolite of 5-fluorouracil, i.e. 5,6 dihydro-5-fluorouracil, instability In blood plasma could be demonstrated clearly. The data of the study showed that the instability can be overcome by proper sample pretreatment procedures. Adjusting the biological samples to pH3.5 and treatment of samples at T=40C Inhibit degradation of 5,6 dihydro-5-fluorouracil.

M3 - Conference contribution

VL - 2

SP - 754

EP - 763

BT - Eighth International Symposium on Capillary Chromatography : Riva del Garda, Italy, May 19-21, 1987.

A2 - Sandra, P.

PB - Huethig

CY - Heidelberg

T2 - 8th International Symposium on Capillary Chromatography, 1987

Y2 - 19 May 1987 through 21 May 1987

ER -

Capillary gas chromatography in cancer research

Abstract

Conference

UN SDGs

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