Capillary gas chromatography in cancer research

E.A. de Bruijn, P.A. Leclercq, A.T. Oosterom, van, U.R. Tjaden, C.J.H. van de Velde, L.M. Brauw, de, T.B.L.W. Marinelli, von

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademic

Abstract

Monitoring of endogenous compounds and xenobiotics including anticancer drugs is of extreme importance in cancer research. The intrOduction of capillary columns allowed selective and sensitive monitoring of anticancer agents in body fluids of cancer patients such as cyclophosphamide and 5-fluorouracil. The use of short capillary columns even allows determination of underivatized drugs; then special care has to be taken for instability of compounds and selectivity towards metabolites. In running a capillary GC assay routinely for a metabolite of 5-fluorouracil, i.e. 5,6 dihydro-5-fluorouracil, instability In blood plasma could be demonstrated clearly. The data of the study showed that the instability can be overcome by proper sample pretreatment procedures. Adjusting the biological samples to pH3.5 and treatment of samples at T=40C Inhibit degradation of 5,6 dihydro-5-fluorouracil.
Original languageEnglish
Title of host publicationEighth International Symposium on Capillary Chromatography : Riva del Garda, Italy, May 19-21, 1987.
EditorsP. Sandra
Place of PublicationHeidelberg
PublisherHuethig
Pages754-763
Volume2
Publication statusPublished - 1987
Event8th International Symposium on Capillary Chromatography, 1987 - Palazzo dei Congressi, Riva del Garda, Italy
Duration: 19 May 198721 May 1987

Conference

Conference8th International Symposium on Capillary Chromatography, 1987
CountryItaly
CityRiva del Garda
Period19/05/8721/05/87

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