Capillary electrophoresis of peptides: analysis of adrenocorticotropic hormone-related fragments

A.A.A.M. vd Goor, P.S.L. Janssen, J.W. Nispen, van, M.J.M. Zeeland, van, F.M. Everaerts

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Abstract

Capillary electrophoresis can be used succesfully to analyse small peptides to give additional information to that obtained using high-performance liquid chromatography (HPLC). The separation of a modified adrenocorticotropic hormone (4-9) fragment (Org 2766) and several of its fragments was investigated using capillary zone electrophoresis. Prediction of migration in aqueous systems using pKa-related data and the migration behaviour using sodium dodecyl sulphate in the buffer are discussed, as is the choice of buffer systems. The electrophoretic patterns are compared with the HPLC separation.
Original languageEnglish
Pages (from-to)379-389
JournalJournal of Chromatography, A
Volume545
Issue number2
DOIs
Publication statusPublished - 1991

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
High performance liquid chromatography
Adrenocorticotropic Hormone
Buffers
High Pressure Liquid Chromatography
Peptides
Electrophoresis
Sodium Dodecyl Sulfate
Org 2766

Cite this

vd Goor, A.A.A.M. ; Janssen, P.S.L. ; Nispen, van, J.W. ; Zeeland, van, M.J.M. ; Everaerts, F.M. / Capillary electrophoresis of peptides: analysis of adrenocorticotropic hormone-related fragments. In: Journal of Chromatography, A. 1991 ; Vol. 545, No. 2. pp. 379-389.
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Capillary electrophoresis of peptides: analysis of adrenocorticotropic hormone-related fragments. / vd Goor, A.A.A.M.; Janssen, P.S.L.; Nispen, van, J.W.; Zeeland, van, M.J.M.; Everaerts, F.M.

In: Journal of Chromatography, A, Vol. 545, No. 2, 1991, p. 379-389.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Capillary electrophoresis of peptides: analysis of adrenocorticotropic hormone-related fragments

AU - vd Goor, A.A.A.M.

AU - Janssen, P.S.L.

AU - Nispen, van, J.W.

AU - Zeeland, van, M.J.M.

AU - Everaerts, F.M.

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AB - Capillary electrophoresis can be used succesfully to analyse small peptides to give additional information to that obtained using high-performance liquid chromatography (HPLC). The separation of a modified adrenocorticotropic hormone (4-9) fragment (Org 2766) and several of its fragments was investigated using capillary zone electrophoresis. Prediction of migration in aqueous systems using pKa-related data and the migration behaviour using sodium dodecyl sulphate in the buffer are discussed, as is the choice of buffer systems. The electrophoretic patterns are compared with the HPLC separation.

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DO - 10.1016/S0021-9673%2801%2988730-6

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JO - Journal of Chromatography, A

JF - Journal of Chromatography, A

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