Capillary electrophoresis of peptides: analysis of adrenocorticotropic hormone-related fragments

A.A.A.M. vd Goor, P.S.L. Janssen, J.W. Nispen, van, M.J.M. Zeeland, van, F.M. Everaerts

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Abstract

Capillary electrophoresis can be used succesfully to analyse small peptides to give additional information to that obtained using high-performance liquid chromatography (HPLC). The separation of a modified adrenocorticotropic hormone (4-9) fragment (Org 2766) and several of its fragments was investigated using capillary zone electrophoresis. Prediction of migration in aqueous systems using pKa-related data and the migration behaviour using sodium dodecyl sulphate in the buffer are discussed, as is the choice of buffer systems. The electrophoretic patterns are compared with the HPLC separation.
Original languageEnglish
Pages (from-to)379-389
JournalJournal of Chromatography, A
Volume545
Issue number2
DOIs
Publication statusPublished - 1991

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