Abstract
Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, yet only little is known about the migration of CSCs in the tumor microenvironment (TME). The presence of oxygen gradients in the TME has been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on breast CSC migration, using a PDMS microfluidic chip. We demonstrate our capability to perform single cell tracking in a stable gradient. Surprisingly, our results indicate a migration direction opposite to the direction reported in a previous study.
| Original language | English |
|---|---|
| Title of host publication | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
| Publisher | Royal Society of Chemistry |
| Pages | 1517-1520 |
| Number of pages | 4 |
| Volume | 3 |
| ISBN (Electronic) | 9781510897571 |
| Publication status | Published - 2018 |
| Event | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 11 Nov 2018 → 15 Nov 2018 https://cbmsociety.org/conferences/microtas2018/ |
Conference
| Conference | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 |
|---|---|
| Country/Territory | Taiwan |
| City | Kaohsiung |
| Period | 11/11/18 → 15/11/18 |
| Internet address |
Funding
This work was supported by ICMS, the Institute of Complex Molecular Systems, and by the European Union through a Marie Curie Career Integration Grant [618623].
Keywords
- Cancer stem cells
- Metastasis
- Microfluidics
- Migration
- Oxygen gradient
- Tumor microenvironment
