Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Recently, cancer stem cells (CSCs) have been proposed as the source of metastases, yet only little is known about the migration of CSCs in the tumor microenvironment (TME). The presence of oxygen gradients in the TME has been linked to directional migration of breast cancer cells. Here, we present a method to study the effect of oxygen gradients on breast CSC migration, using a PDMS microfluidic chip. We demonstrate our capability to perform single cell tracking in a stable gradient. Surprisingly, our results indicate a migration direction opposite to the direction reported in a previous study.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1517-1520
Number of pages4
Volume3
ISBN (Electronic)9781510897571
Publication statusPublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 11 Nov 201815 Nov 2018

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan
CityKaohsiung
Period11/11/1815/11/18

Keywords

  • Cancer stem cells
  • Metastasis
  • Microfluidics
  • Migration
  • Oxygen gradient
  • Tumor microenvironment

Fingerprint Dive into the research topics of 'Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device'. Together they form a unique fingerprint.

  • Cite this

    Sleeboom, J. J. F., Sahlgren, C. M., & Den Toonder, J. M. J. (2018). Cancer stem cell migration in an oxygen gradient characterized using a microfluidic device. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (Vol. 3, pp. 1517-1520). Chemical and Biological Microsystems Society.