Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system

H. Eslami Amirabadi; M. Tuerlings; S. SahebAli; R. Luttge; C.C. van Donkelaar; J. W.M. Martens; J.M.J. den Toonder

Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system

H. Eslami Amirabadi, M. Tuerlings, S. SahebAli, R. Luttge, C.C. van Donkelaar, J. W.M. Martens, J.M.J. den Toonder

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

Different E-cadherin status in breast cancer is associated with different invasion abilities. However, a relevant in vitro study to study this is still missing from the literature. We developed a microfluidic system to characterize the invasion of three breast cancer cell lines with distinct E-cadherin status in 3D under the influence of a chemotactic gradient. We showed that this system can be used to capture the cells’ migration ability and invasion pattern. Using a perfusion system to control chemotactic gradients and integration of a 3D extracellular matrix, we could mimic two essential components of the tumor microenvironment during cancer invasion.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Publisher	Royal Society of Chemistry
Pages	1658-1660
Number of pages	3
Volume	3
ISBN (Electronic)	9781510897571
Publication status	Published - 1 Jan 2018
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung Exhibition Center, Kaohsiung, Taiwan Duration: 11 Nov 2018 → 15 Nov 2018 https://cbmsociety.org/conferences/microtas2018/

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/Territory	Taiwan
City	Kaohsiung
Period	11/11/18 → 15/11/18
Internet address	https://cbmsociety.org/conferences/microtas2018/

Keywords

Breast cancer cell invasion
Chemotactic gradient
E-cadherin
Invasion mode
Microfluidics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Eslami Amirabadi, H., Tuerlings, M., SahebAli, S., Luttge, R., van Donkelaar, C. C., Martens, J. W. M., & den Toonder, J. M. J. (2018). Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 (Vol. 3, pp. 1658-1660). Royal Society of Chemistry.

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title = "Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system",

abstract = "Different E-cadherin status in breast cancer is associated with different invasion abilities. However, a relevant in vitro study to study this is still missing from the literature. We developed a microfluidic system to characterize the invasion of three breast cancer cell lines with distinct E-cadherin status in 3D under the influence of a chemotactic gradient. We showed that this system can be used to capture the cells{\textquoteright} migration ability and invasion pattern. Using a perfusion system to control chemotactic gradients and integration of a 3D extracellular matrix, we could mimic two essential components of the tumor microenvironment during cancer invasion.",

keywords = "Breast cancer cell invasion, Chemotactic gradient, E-cadherin, Invasion mode, Microfluidics",

author = "{Eslami Amirabadi}, H. and M. Tuerlings and S. SahebAli and R. Luttge and {van Donkelaar}, C.C. and Martens, {J. W.M.} and {den Toonder}, J.M.J.",

year = "2018",

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language = "English",

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booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018",

publisher = "Royal Society of Chemistry",

address = "United Kingdom",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 ; Conference date: 11-11-2018 Through 15-11-2018",

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Eslami Amirabadi, H, Tuerlings, M, SahebAli, S, Luttge, R , van Donkelaar, CC, Martens, JWM & den Toonder, JMJ 2018, Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. vol. 3, Royal Society of Chemistry, pp. 1658-1660, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018, Kaohsiung, Taiwan, 11/11/18.

Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system. / Eslami Amirabadi, H.; Tuerlings, M.; SahebAli, S. et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Vol. 3 Royal Society of Chemistry, 2018. p. 1658-1660.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system

AU - Eslami Amirabadi, H.

AU - Tuerlings, M.

AU - SahebAli, S.

AU - Luttge, R.

AU - van Donkelaar, C.C.

AU - Martens, J. W.M.

AU - den Toonder, J.M.J.

PY - 2018/1/1

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N2 - Different E-cadherin status in breast cancer is associated with different invasion abilities. However, a relevant in vitro study to study this is still missing from the literature. We developed a microfluidic system to characterize the invasion of three breast cancer cell lines with distinct E-cadherin status in 3D under the influence of a chemotactic gradient. We showed that this system can be used to capture the cells’ migration ability and invasion pattern. Using a perfusion system to control chemotactic gradients and integration of a 3D extracellular matrix, we could mimic two essential components of the tumor microenvironment during cancer invasion.

AB - Different E-cadherin status in breast cancer is associated with different invasion abilities. However, a relevant in vitro study to study this is still missing from the literature. We developed a microfluidic system to characterize the invasion of three breast cancer cell lines with distinct E-cadherin status in 3D under the influence of a chemotactic gradient. We showed that this system can be used to capture the cells’ migration ability and invasion pattern. Using a perfusion system to control chemotactic gradients and integration of a 3D extracellular matrix, we could mimic two essential components of the tumor microenvironment during cancer invasion.

KW - Breast cancer cell invasion

KW - Chemotactic gradient

KW - E-cadherin

KW - Invasion mode

KW - Microfluidics

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SP - 1658

EP - 1660

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

PB - Royal Society of Chemistry

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

Y2 - 11 November 2018 through 15 November 2018

ER -

Eslami Amirabadi H, Tuerlings M, SahebAli S, Luttge R , van Donkelaar CC, Martens JWM et al. Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018. Vol. 3. Royal Society of Chemistry. 2018. p. 1658-1660

Breast cancer cells with distinct E-cadherin status exhibit different 3-D invasion behavior in a novel microfluidic system

Abstract

Conference

Keywords

UN SDGs

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