BMP-silk composite matrices heal critically sized femoral defects

C. Kirker-Head, V. Karageorgiou, S. Hofmann, R. Fajardo, O. Betz, H.P. Merkle, M. Hilbe, B. Rechenberg, von, J. McCool, L. Abrahamsen, A. Nazarian, E. Cory, M. Curtis, D.L. Kaplan, L. Meinel

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Abstract

Clinical drawbacks of bone grafting prompt the search for alternative bone augmentation technologies such as use of growth and differentiation factors, gene therapy, and cell therapy. Osteopromotive matrices are frequently employed for the local delivery and controlled release of these augmentation agents. Some matrices also provide an osteoconductive scaffold to support new bone growth. In this study, silkworm-derived silk fibroin was evaluated as an osteoconductive matrix for healing critical sized mid-femoral segmental defects in nude rats. Four treatment groups were assessed over eight weeks: silk scaffolds (SS) with recombinant human BMP-2 (rhBMP-2) and human mesenchymal stem cells (HMSC) that had been pre-differentiated along an osteoblastic lineage ex vivo (Group I; pdHMSC/rhBMP-2/SS); SS with rhBMP-2 and undifferentiated HMSCs (Group II; udHMSC/rhBMP-2/SS); SS and rhBMP-2 alone (Group III; rhBMP-2/SS); and empty defects (Group IV). Bi-weekly radiographs revealed a progressive and similar increase in Group I-III mean defect mineralization through post-operative week (POW) 8. Radiographs, dual energy x-ray absorptiometry, and micro-computed tomography confirmed that Groups I-III exhibited similar substantial and significantly (p
Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalBone
Volume41
Issue number2
DOIs
Publication statusPublished - 2007

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Silk
Thigh
Growth Differentiation Factors
Fibroins
Nude Rats
Bombyx
Bone Transplantation
Bone Development
Cell- and Tissue-Based Therapy
Mesenchymal Stromal Cells
Genetic Therapy
Tomography
X-Rays
Technology
Bone and Bones

Cite this

Kirker-Head, C., Karageorgiou, V., Hofmann, S., Fajardo, R., Betz, O., Merkle, H. P., ... Meinel, L. (2007). BMP-silk composite matrices heal critically sized femoral defects. Bone, 41(2), 247-255. https://doi.org/10.1016/j.bone.2007.04.186
Kirker-Head, C. ; Karageorgiou, V. ; Hofmann, S. ; Fajardo, R. ; Betz, O. ; Merkle, H.P. ; Hilbe, M. ; Rechenberg, von, B. ; McCool, J. ; Abrahamsen, L. ; Nazarian, A. ; Cory, E. ; Curtis, M. ; Kaplan, D.L. ; Meinel, L. / BMP-silk composite matrices heal critically sized femoral defects. In: Bone. 2007 ; Vol. 41, No. 2. pp. 247-255.
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abstract = "Clinical drawbacks of bone grafting prompt the search for alternative bone augmentation technologies such as use of growth and differentiation factors, gene therapy, and cell therapy. Osteopromotive matrices are frequently employed for the local delivery and controlled release of these augmentation agents. Some matrices also provide an osteoconductive scaffold to support new bone growth. In this study, silkworm-derived silk fibroin was evaluated as an osteoconductive matrix for healing critical sized mid-femoral segmental defects in nude rats. Four treatment groups were assessed over eight weeks: silk scaffolds (SS) with recombinant human BMP-2 (rhBMP-2) and human mesenchymal stem cells (HMSC) that had been pre-differentiated along an osteoblastic lineage ex vivo (Group I; pdHMSC/rhBMP-2/SS); SS with rhBMP-2 and undifferentiated HMSCs (Group II; udHMSC/rhBMP-2/SS); SS and rhBMP-2 alone (Group III; rhBMP-2/SS); and empty defects (Group IV). Bi-weekly radiographs revealed a progressive and similar increase in Group I-III mean defect mineralization through post-operative week (POW) 8. Radiographs, dual energy x-ray absorptiometry, and micro-computed tomography confirmed that Groups I-III exhibited similar substantial and significantly (p",
author = "C. Kirker-Head and V. Karageorgiou and S. Hofmann and R. Fajardo and O. Betz and H.P. Merkle and M. Hilbe and {Rechenberg, von}, B. and J. McCool and L. Abrahamsen and A. Nazarian and E. Cory and M. Curtis and D.L. Kaplan and L. Meinel",
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Kirker-Head, C, Karageorgiou, V, Hofmann, S, Fajardo, R, Betz, O, Merkle, HP, Hilbe, M, Rechenberg, von, B, McCool, J, Abrahamsen, L, Nazarian, A, Cory, E, Curtis, M, Kaplan, DL & Meinel, L 2007, 'BMP-silk composite matrices heal critically sized femoral defects', Bone, vol. 41, no. 2, pp. 247-255. https://doi.org/10.1016/j.bone.2007.04.186

BMP-silk composite matrices heal critically sized femoral defects. / Kirker-Head, C.; Karageorgiou, V.; Hofmann, S.; Fajardo, R.; Betz, O.; Merkle, H.P.; Hilbe, M.; Rechenberg, von, B.; McCool, J.; Abrahamsen, L.; Nazarian, A.; Cory, E.; Curtis, M.; Kaplan, D.L.; Meinel, L.

In: Bone, Vol. 41, No. 2, 2007, p. 247-255.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - BMP-silk composite matrices heal critically sized femoral defects

AU - Kirker-Head, C.

AU - Karageorgiou, V.

AU - Hofmann, S.

AU - Fajardo, R.

AU - Betz, O.

AU - Merkle, H.P.

AU - Hilbe, M.

AU - Rechenberg, von, B.

AU - McCool, J.

AU - Abrahamsen, L.

AU - Nazarian, A.

AU - Cory, E.

AU - Curtis, M.

AU - Kaplan, D.L.

AU - Meinel, L.

PY - 2007

Y1 - 2007

N2 - Clinical drawbacks of bone grafting prompt the search for alternative bone augmentation technologies such as use of growth and differentiation factors, gene therapy, and cell therapy. Osteopromotive matrices are frequently employed for the local delivery and controlled release of these augmentation agents. Some matrices also provide an osteoconductive scaffold to support new bone growth. In this study, silkworm-derived silk fibroin was evaluated as an osteoconductive matrix for healing critical sized mid-femoral segmental defects in nude rats. Four treatment groups were assessed over eight weeks: silk scaffolds (SS) with recombinant human BMP-2 (rhBMP-2) and human mesenchymal stem cells (HMSC) that had been pre-differentiated along an osteoblastic lineage ex vivo (Group I; pdHMSC/rhBMP-2/SS); SS with rhBMP-2 and undifferentiated HMSCs (Group II; udHMSC/rhBMP-2/SS); SS and rhBMP-2 alone (Group III; rhBMP-2/SS); and empty defects (Group IV). Bi-weekly radiographs revealed a progressive and similar increase in Group I-III mean defect mineralization through post-operative week (POW) 8. Radiographs, dual energy x-ray absorptiometry, and micro-computed tomography confirmed that Groups I-III exhibited similar substantial and significantly (p

AB - Clinical drawbacks of bone grafting prompt the search for alternative bone augmentation technologies such as use of growth and differentiation factors, gene therapy, and cell therapy. Osteopromotive matrices are frequently employed for the local delivery and controlled release of these augmentation agents. Some matrices also provide an osteoconductive scaffold to support new bone growth. In this study, silkworm-derived silk fibroin was evaluated as an osteoconductive matrix for healing critical sized mid-femoral segmental defects in nude rats. Four treatment groups were assessed over eight weeks: silk scaffolds (SS) with recombinant human BMP-2 (rhBMP-2) and human mesenchymal stem cells (HMSC) that had been pre-differentiated along an osteoblastic lineage ex vivo (Group I; pdHMSC/rhBMP-2/SS); SS with rhBMP-2 and undifferentiated HMSCs (Group II; udHMSC/rhBMP-2/SS); SS and rhBMP-2 alone (Group III; rhBMP-2/SS); and empty defects (Group IV). Bi-weekly radiographs revealed a progressive and similar increase in Group I-III mean defect mineralization through post-operative week (POW) 8. Radiographs, dual energy x-ray absorptiometry, and micro-computed tomography confirmed that Groups I-III exhibited similar substantial and significantly (p

U2 - 10.1016/j.bone.2007.04.186

DO - 10.1016/j.bone.2007.04.186

M3 - Article

VL - 41

SP - 247

EP - 255

JO - Bone

JF - Bone

SN - 8756-3282

IS - 2

ER -

Kirker-Head C, Karageorgiou V, Hofmann S, Fajardo R, Betz O, Merkle HP et al. BMP-silk composite matrices heal critically sized femoral defects. Bone. 2007;41(2):247-255. https://doi.org/10.1016/j.bone.2007.04.186