Bioseparations in aqueous micellar systems based on excluded-volume interactions

D. Roosmalen, van; M.P.J. Dohmen-Speelmans; C.H.J.T. Dietz; L.J.P. Broeke, van den; L.A.M. Wielen, van der; J.T.F. Keurentjes

doi:10.1205/fbp.05159

Bioseparations in aqueous micellar systems based on excluded-volume interactions

D. Roosmalen, van, M.P.J. Dohmen-Speelmans, C.H.J.T. Dietz, L.J.P. Broeke, van den, L.A.M. Wielen, van der, J.T.F. Keurentjes

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Abstract

Protein partitioning based on micellar-induced excluded-vol. interactions is studied for different aq. nonionic micellar systems. In particular, protein partitioning in the traditional aq. micellar two-phase system is compared with a system where a gel phase is combined with a micellar phase and with a membrane system with micelles at the feed side of the membrane. A two-phase system is created using cylindrically-shaped n-decyl tetra(ethylene oxide) (C10E4) micelles that are too large to diffuse into the gel-bead or across the membrane. Results are reported for the partition coeff. of single-component protein systems with myoglobin, ovalbumin, and BSA, and for binary protein mixts. The individual protein partition coeffs. in the mixts. follow the same protein concn. dependence as the partition coeff. obtained for the single-component protein solns. [on SciFinder (R)]

Original language	English
Pages (from-to)	51-58
Number of pages	8
Journal	Food and Bioproducts Processing
Volume	84
Issue number	C1
DOIs	https://doi.org/10.1205/fbp.05159
Publication status	Published - 2006

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title = "Bioseparations in aqueous micellar systems based on excluded-volume interactions",

abstract = "Protein partitioning based on micellar-induced excluded-vol. interactions is studied for different aq. nonionic micellar systems. In particular, protein partitioning in the traditional aq. micellar two-phase system is compared with a system where a gel phase is combined with a micellar phase and with a membrane system with micelles at the feed side of the membrane. A two-phase system is created using cylindrically-shaped n-decyl tetra(ethylene oxide) (C10E4) micelles that are too large to diffuse into the gel-bead or across the membrane. Results are reported for the partition coeff. of single-component protein systems with myoglobin, ovalbumin, and BSA, and for binary protein mixts. The individual protein partition coeffs. in the mixts. follow the same protein concn. dependence as the partition coeff. obtained for the single-component protein solns. [on SciFinder (R)]",

author = "{Roosmalen, van}, D. and M.P.J. Dohmen-Speelmans and C.H.J.T. Dietz and {Broeke, van den}, L.J.P. and {Wielen, van der}, L.A.M. and J.T.F. Keurentjes",

year = "2006",

doi = "10.1205/fbp.05159",

language = "English",

volume = "84",

pages = "51--58",

journal = "Food and Bioproducts Processing",

issn = "0960-3085",

publisher = "Institution of Chemical Engineers",

number = "C1",

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TY - JOUR

T1 - Bioseparations in aqueous micellar systems based on excluded-volume interactions

AU - Roosmalen, van, D.

AU - Dohmen-Speelmans, M.P.J.

AU - Dietz, C.H.J.T.

AU - Broeke, van den, L.J.P.

AU - Wielen, van der, L.A.M.

AU - Keurentjes, J.T.F.

PY - 2006

Y1 - 2006

N2 - Protein partitioning based on micellar-induced excluded-vol. interactions is studied for different aq. nonionic micellar systems. In particular, protein partitioning in the traditional aq. micellar two-phase system is compared with a system where a gel phase is combined with a micellar phase and with a membrane system with micelles at the feed side of the membrane. A two-phase system is created using cylindrically-shaped n-decyl tetra(ethylene oxide) (C10E4) micelles that are too large to diffuse into the gel-bead or across the membrane. Results are reported for the partition coeff. of single-component protein systems with myoglobin, ovalbumin, and BSA, and for binary protein mixts. The individual protein partition coeffs. in the mixts. follow the same protein concn. dependence as the partition coeff. obtained for the single-component protein solns. [on SciFinder (R)]

AB - Protein partitioning based on micellar-induced excluded-vol. interactions is studied for different aq. nonionic micellar systems. In particular, protein partitioning in the traditional aq. micellar two-phase system is compared with a system where a gel phase is combined with a micellar phase and with a membrane system with micelles at the feed side of the membrane. A two-phase system is created using cylindrically-shaped n-decyl tetra(ethylene oxide) (C10E4) micelles that are too large to diffuse into the gel-bead or across the membrane. Results are reported for the partition coeff. of single-component protein systems with myoglobin, ovalbumin, and BSA, and for binary protein mixts. The individual protein partition coeffs. in the mixts. follow the same protein concn. dependence as the partition coeff. obtained for the single-component protein solns. [on SciFinder (R)]

U2 - 10.1205/fbp.05159

DO - 10.1205/fbp.05159

M3 - Article

SN - 0960-3085

VL - 84

SP - 51

EP - 58

JO - Food and Bioproducts Processing

JF - Food and Bioproducts Processing

IS - C1

ER -

Bioseparations in aqueous micellar systems based on excluded-volume interactions

Abstract

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