Bimodal Targeting of Human Leukocytes by Fc- And CpG-Decorated Polymersomes to Tune Immune Induction

Lucille F. van Beek (Corresponding author), Pascal L.W. Welzen, Lisa U. Teufel, Irma Joosten, Dimitri A. Diavatopoulos, Jan van Hest, Marien I. de Jonge

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Abstract

The use of well-defined nanovesicles composed of amphiphilic block copolymers (polymersomes) for delivery of adjuvants and antigens is a promising strategy for vaccine development. However, the potency of nanoparticle vaccines depends on efficient interaction with and activation of cells involved in antigen presentation, which can be achieved by targeting cellular receptors. Here, we showed that the Fc fragment display on the polymersome surface resulted in markedly improved interactions with granulocytes, monocytes, and NK cells, while for "naked"polymersomes, virtually no binding to leukocytes was observed. Moreover, CpG-decorated polymersomes were found to also interact with T and/or B cells. Interestingly, whole blood stimulations with Fc fragment and CpG-decorated polymersomes induced interleukin (IL)-6, IL-8, and TNF-α production, while naked polymersomes did not induce any cytokine production. In conclusion, specific immune induction by polymersomes can be controlled using bimodal targeting of different immune receptors, which is an essential feature for targeted vaccine delivery.

Original languageEnglish
Pages (from-to)4422-4433
Number of pages12
JournalBiomacromolecules
Volume22
Issue number10
DOIs
Publication statusPublished - 11 Oct 2021

Bibliographical note

Funding Information:
This study was supported by “NWO TTW Perspectief Programma”, “A technology center for Bacterial Vaccines (BacVactory)”.

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