Abstract
In the last decade, point accumulation for imaging in nanoscale topography (PAINT) has emerged as a versatile tool for single-molecule localization microscopy (SMLM). Currently, DNA-PAINT is the most widely used, in which a transient stochastically binding DNA docking-imaging pair is used to reconstruct specific characteristics of biological or synthetic materials on a single-molecule level. Slowly, the need for PAINT probes that are not dependent on DNA has emerged. These probes can be based on (i) endogenous interactions, (ii) engineered binders, (iii) fusion proteins, or (iv) synthetic molecules and provide complementary applications for SMLM. Therefore, researchers have been expanding the PAINT toolbox with new probes. In this review, we provide an overview of the currently existing probes that go beyond DNA and their applications and challenges.
| Original language | English |
|---|---|
| Pages (from-to) | 8332-8342 |
| Number of pages | 11 |
| Journal | Chemical Communications, ChemComm |
| Volume | 59 |
| Issue number | 54 |
| DOIs | |
| Publication status | Published - 4 Jul 2023 |
Bibliographical note
Funding Information:This work is supported by the Dutch Research Council, through VIDI grant 192.028 to L. A. and M. M. E. T. and VENI grant 202.220 to R. P. T. All schematics were created using Biorender.com.
Funding
This work is supported by the Dutch Research Council, through VIDI grant 192.028 to L. A. and M. M. E. T. and VENI grant 202.220 to R. P. T. All schematics were created using Biorender.com.
Keywords
- Microscopy, Fluorescence/methods
- DNA/chemistry
- Nanotechnology/methods
- Single Molecule Imaging/methods