Assessing bioink shape fidelity to aid material development in 3D bioprinting

A. Ribeiro, M.M. Blokzijl, R. Levato, C.W. Visser, M. Castilho, W.E. Hennink, T. Vermonden, J. Malda

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)

Abstract

During extrusion-based bioprinting, the deposited bioink filaments are subjected to deformations, such as collapse of overhanging filaments, which compromises the ability to stack several layers of bioink, and fusion between adjacent filaments, which compromises the resolution and maintenance of a desired pore structure. When developing new bioinks, approaches to assess their shape fidelity after printing would be beneficial to evaluate the degree of deformation of the deposited filament and to estimate how similar the final printed construct would be to the design. However, shape fidelity has been prevalently assessed qualitatively through visual inspection after printing, hampering the direct comparison of the printability of different bioinks. In this technical note, we propose a quantitative evaluation for shape fidelity of bioinks based on testing the filament collapse on overhanging structures and the filament fusion of parallel printed strands. Both tests were applied on a hydrogel platform based on poloxamer 407 and poly(ethylene glycol) blends, providing a library of hydrogels with different yield stresses. The presented approach is an easy way to assess bioink shape fidelity, applicable to any filament-based bioprinting system and able to quantitatively evaluate this aspect of printability, based on the degree of deformation of the printed filament. In addition, we built a simple theoretical model that relates filament collapse with bioink yield stress. The results of both shape fidelity tests underline the role of yield stress as one of the parameters influencing the printability of a bioink. The presented quantitative evaluation will allow for reproducible comparisons between different bioink platforms.

Original languageEnglish
Article number014102
Number of pages10
JournalBiofabrication
Volume10
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Bioprinting
Printing
Yield stress
Poloxamer
Hydrogels
Ethylene Glycol
Hydrogel
Fusion reactions
Theoretical Models
Maintenance
Pore structure
Polyethylene glycols
Extrusion
Inspection
Testing

Keywords

  • bioink
  • bioprinting
  • extrusion
  • hydrogel
  • shape fidelity
  • yield stress

Cite this

Ribeiro, A., Blokzijl, M. M., Levato, R., Visser, C. W., Castilho, M., Hennink, W. E., ... Malda, J. (2018). Assessing bioink shape fidelity to aid material development in 3D bioprinting. Biofabrication, 10(1), [014102]. https://doi.org/10.1088/1758-5090/aa90e2
Ribeiro, A. ; Blokzijl, M.M. ; Levato, R. ; Visser, C.W. ; Castilho, M. ; Hennink, W.E. ; Vermonden, T. ; Malda, J. / Assessing bioink shape fidelity to aid material development in 3D bioprinting. In: Biofabrication. 2018 ; Vol. 10, No. 1.
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Ribeiro, A, Blokzijl, MM, Levato, R, Visser, CW, Castilho, M, Hennink, WE, Vermonden, T & Malda, J 2018, 'Assessing bioink shape fidelity to aid material development in 3D bioprinting', Biofabrication, vol. 10, no. 1, 014102. https://doi.org/10.1088/1758-5090/aa90e2

Assessing bioink shape fidelity to aid material development in 3D bioprinting. / Ribeiro, A.; Blokzijl, M.M.; Levato, R.; Visser, C.W.; Castilho, M.; Hennink, W.E.; Vermonden, T.; Malda, J.

In: Biofabrication, Vol. 10, No. 1, 014102, 01.01.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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