Assembly of protein stacks with in situ synthesized nanoparticle cargo

Sesha Manuguri, Kyle Webster, N. Amy Yewdall, Yiran An, Hari Venugopal, Vaibhav Bhugra, Adrian Turner, Laura J. Domigan, Juliet A. Gerrard, David E. Williams, Jenny Malmström

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

The ability of proteins to form hierarchical structures through self-assembly provides an opportunity to synthesize and organize nanoparticles. Ordered nanoparticle assemblies are a subject of widespread interest due to the potential to harness their emergent functions. In this work, the toroidal-shaped form of the protein peroxiredoxin, which has a pore size of 7 nm, was used to organize iron oxyhydroxide nanoparticles. Iron in the form of Fe2+ was sequestered into the central cavity of the toroid ring using metal-binding sites engineered there and then hydrolyzed to form iron oxyhydroxide particles bound into the protein pore. By precise manipulation of the pH, the mineralized toroids were organized into stacks confining one-dimensional nanoparticle assemblies. We report the formation and the procedures leading to the formation of such nanostructures and their characterization by chromatography and microscopy. Electrostatic force microscopy clearly revealed the formation of iron-containing nanorods as a result of the self-assembly of the iron-loaded protein. This research bodes well for the use of peroxiredoxin as a template with which to form nanowires and structures for electronic and magnetic applications.

LanguageEnglish
Pages5138-5145
Number of pages8
JournalNano Letters
Volume18
Issue number8
DOIs
StatePublished - 8 Aug 2018

Fingerprint

cargo
assembly
Iron
Nanoparticles
proteins
Proteins
Peroxiredoxins
iron
nanoparticles
toroids
Self assembly
Microscopic examination
assemblies
self assembly
Porins
Electrostatic force
microscopy
porosity
harnesses
Chromatography

Keywords

  • electrostatic force microscopy
  • iron-oxide mineralization
  • nanoparticle organization
  • peroxiredoxin
  • Protein nanotechnology

Cite this

Manuguri, S., Webster, K., Yewdall, N. A., An, Y., Venugopal, H., Bhugra, V., ... Malmström, J. (2018). Assembly of protein stacks with in situ synthesized nanoparticle cargo. Nano Letters, 18(8), 5138-5145. DOI: 10.1021/acs.nanolett.8b02055
Manuguri, Sesha ; Webster, Kyle ; Yewdall, N. Amy ; An, Yiran ; Venugopal, Hari ; Bhugra, Vaibhav ; Turner, Adrian ; Domigan, Laura J. ; Gerrard, Juliet A. ; Williams, David E. ; Malmström, Jenny. / Assembly of protein stacks with in situ synthesized nanoparticle cargo. In: Nano Letters. 2018 ; Vol. 18, No. 8. pp. 5138-5145
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Manuguri, S, Webster, K, Yewdall, NA, An, Y, Venugopal, H, Bhugra, V, Turner, A, Domigan, LJ, Gerrard, JA, Williams, DE & Malmström, J 2018, 'Assembly of protein stacks with in situ synthesized nanoparticle cargo' Nano Letters, vol. 18, no. 8, pp. 5138-5145. DOI: 10.1021/acs.nanolett.8b02055

Assembly of protein stacks with in situ synthesized nanoparticle cargo. / Manuguri, Sesha; Webster, Kyle; Yewdall, N. Amy; An, Yiran; Venugopal, Hari; Bhugra, Vaibhav; Turner, Adrian; Domigan, Laura J.; Gerrard, Juliet A.; Williams, David E.; Malmström, Jenny.

In: Nano Letters, Vol. 18, No. 8, 08.08.2018, p. 5138-5145.

Research output: Contribution to journalArticleAcademicpeer-review

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Manuguri S, Webster K, Yewdall NA, An Y, Venugopal H, Bhugra V et al. Assembly of protein stacks with in situ synthesized nanoparticle cargo. Nano Letters. 2018 Aug 8;18(8):5138-5145. Available from, DOI: 10.1021/acs.nanolett.8b02055