Abstract
14-3-3 proteins are adaptor elements in intracellular signaling pathways. Recently, this protein family has been identified as a relevant therapeutic target involved in many human diseases. Therefore, identification of 14-3-3 proteins in biological systems is very important. Two cationic peptide-based probes are reported for the fluorescence detection of 14-3-3 proteins at physiological pH. The design of these probes consists of two symmetric peptidic arms equipped with a guanidiniocarbonyl pyrrole moiety (an arginine mimetic aka GCP), and an environment-sensitive amino-naphthalimide fluorophore as a third arm. These peptide sequences also contain lysine and phenylalanine/tryptophan amino acids for additional charge-charge and hydrophobic interactions. Both probes show high affinity and sensitivity for the 14-3-3 family, as well as good selectivity against other relevant biological proteins and ions.
| Original language | English |
|---|---|
| Pages (from-to) | 4359-4363 |
| Number of pages | 5 |
| Journal | Organic & Biomolecular Chemistry |
| Volume | 17 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2 Apr 2019 |
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Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins. / Maity, Debabrata; Gigante, Alba; Sánchez-Murcia, Pedro A.; Sijbesma, Eline; Li, Mao; Bier, David; Mosel, Stefanie; Knauer, Shirley; Ottmann, Christian (Corresponding author); Schmuck, Carsten (Corresponding author).
In: Organic & Biomolecular Chemistry, Vol. 17, No. 17, 02.04.2019, p. 4359-4363.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins
AU - Maity, Debabrata
AU - Gigante, Alba
AU - Sánchez-Murcia, Pedro A.
AU - Sijbesma, Eline
AU - Li, Mao
AU - Bier, David
AU - Mosel, Stefanie
AU - Knauer, Shirley
AU - Ottmann, Christian
AU - Schmuck, Carsten
PY - 2019/4/2
Y1 - 2019/4/2
N2 - 14-3-3 proteins are adaptor elements in intracellular signaling pathways. Recently, this protein family has been identified as a relevant therapeutic target involved in many human diseases. Therefore, identification of 14-3-3 proteins in biological systems is very important. Two cationic peptide-based probes are reported for the fluorescence detection of 14-3-3 proteins at physiological pH. The design of these probes consists of two symmetric peptidic arms equipped with a guanidiniocarbonyl pyrrole moiety (an arginine mimetic aka GCP), and an environment-sensitive amino-naphthalimide fluorophore as a third arm. These peptide sequences also contain lysine and phenylalanine/tryptophan amino acids for additional charge-charge and hydrophobic interactions. Both probes show high affinity and sensitivity for the 14-3-3 family, as well as good selectivity against other relevant biological proteins and ions.
AB - 14-3-3 proteins are adaptor elements in intracellular signaling pathways. Recently, this protein family has been identified as a relevant therapeutic target involved in many human diseases. Therefore, identification of 14-3-3 proteins in biological systems is very important. Two cationic peptide-based probes are reported for the fluorescence detection of 14-3-3 proteins at physiological pH. The design of these probes consists of two symmetric peptidic arms equipped with a guanidiniocarbonyl pyrrole moiety (an arginine mimetic aka GCP), and an environment-sensitive amino-naphthalimide fluorophore as a third arm. These peptide sequences also contain lysine and phenylalanine/tryptophan amino acids for additional charge-charge and hydrophobic interactions. Both probes show high affinity and sensitivity for the 14-3-3 family, as well as good selectivity against other relevant biological proteins and ions.
UR - http://www.scopus.com/inward/record.url?scp=85064967221&partnerID=8YFLogxK
U2 - 10.1039/c9ob00620f
DO - 10.1039/c9ob00620f
M3 - Article
C2 - 30977756
AN - SCOPUS:85064967221
VL - 17
SP - 4359
EP - 4363
JO - Organic & Biomolecular Chemistry
JF - Organic & Biomolecular Chemistry
SN - 1477-0520
IS - 17
ER -