Applying ceramic nanoporous microneedle arrays as a transport interface in egg plants and an ex-vivo human skin model

Michel Verhoeven, S.N. Bystrova, L. Winnubst, H. Qureshi, T.D. Gruijl, de, R.J. Scheper, R. Luttge

Research output: Contribution to journalArticleAcademicpeer-review

24 Citations (Scopus)

Abstract

Nanoporous microneedle arrays from Al 2O 3 were fabricated via a micromolding process using a PDMS mold generated via a double replication process from a SU-8/Si-master as a template. Hg-porosity measurements showed that the porosity obtained was a function of the temperature used for sintering, resulting in an average pore diameter of 80 nm between 1300 and 1500 °C. Using egg plants it was shown that these nanoporous needles allowed both the delivery of substances, and the extraction of compounds. Subsequently, the delivery of compounds has been evaluated in an ex vivo human skin model using a microneedle array saturated with a labeled monoclonal against a specific marker, DC-SIGN, which is representative for dendritic cells when activated by an antigen. By the latter, it was demonstrated that ceramic nanoporous microneedle arrays are potentially useful for the delivery of vaccines. © 2012 Elsevier B.V. All rights reserved.
LanguageEnglish
Pages659-662
Number of pages4
JournalMicroelectronic Engineering
Volume98
DOIs
StatePublished - 2012
Externally publishedYes

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eggs
delivery
Skin
Porosity
ceramics
porosity
Vaccines
Antigens
Needles
vaccines
Sintering
antigens
needles
markers
sintering
templates
direct current
cells
Temperature
temperature

Cite this

Verhoeven, M., Bystrova, S. N., Winnubst, L., Qureshi, H., Gruijl, de, T. D., Scheper, R. J., & Luttge, R. (2012). Applying ceramic nanoporous microneedle arrays as a transport interface in egg plants and an ex-vivo human skin model. Microelectronic Engineering, 98, 659-662. DOI: 10.1016/j.mee.2012.07.022
Verhoeven, Michel ; Bystrova, S.N. ; Winnubst, L. ; Qureshi, H. ; Gruijl, de, T.D. ; Scheper, R.J. ; Luttge, R./ Applying ceramic nanoporous microneedle arrays as a transport interface in egg plants and an ex-vivo human skin model. In: Microelectronic Engineering. 2012 ; Vol. 98. pp. 659-662
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abstract = "Nanoporous microneedle arrays from Al 2O 3 were fabricated via a micromolding process using a PDMS mold generated via a double replication process from a SU-8/Si-master as a template. Hg-porosity measurements showed that the porosity obtained was a function of the temperature used for sintering, resulting in an average pore diameter of 80 nm between 1300 and 1500 °C. Using egg plants it was shown that these nanoporous needles allowed both the delivery of substances, and the extraction of compounds. Subsequently, the delivery of compounds has been evaluated in an ex vivo human skin model using a microneedle array saturated with a labeled monoclonal against a specific marker, DC-SIGN, which is representative for dendritic cells when activated by an antigen. By the latter, it was demonstrated that ceramic nanoporous microneedle arrays are potentially useful for the delivery of vaccines. {\circledC} 2012 Elsevier B.V. All rights reserved.",
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Applying ceramic nanoporous microneedle arrays as a transport interface in egg plants and an ex-vivo human skin model. / Verhoeven, Michel; Bystrova, S.N.; Winnubst, L.; Qureshi, H.; Gruijl, de, T.D.; Scheper, R.J.; Luttge, R.

In: Microelectronic Engineering, Vol. 98, 2012, p. 659-662.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Applying ceramic nanoporous microneedle arrays as a transport interface in egg plants and an ex-vivo human skin model

AU - Verhoeven,Michel

AU - Bystrova,S.N.

AU - Winnubst,L.

AU - Qureshi,H.

AU - Gruijl, de,T.D.

AU - Scheper,R.J.

AU - Luttge,R.

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AB - Nanoporous microneedle arrays from Al 2O 3 were fabricated via a micromolding process using a PDMS mold generated via a double replication process from a SU-8/Si-master as a template. Hg-porosity measurements showed that the porosity obtained was a function of the temperature used for sintering, resulting in an average pore diameter of 80 nm between 1300 and 1500 °C. Using egg plants it was shown that these nanoporous needles allowed both the delivery of substances, and the extraction of compounds. Subsequently, the delivery of compounds has been evaluated in an ex vivo human skin model using a microneedle array saturated with a labeled monoclonal against a specific marker, DC-SIGN, which is representative for dendritic cells when activated by an antigen. By the latter, it was demonstrated that ceramic nanoporous microneedle arrays are potentially useful for the delivery of vaccines. © 2012 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.mee.2012.07.022

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M3 - Article

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T2 - Microelectronic Engineering

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ER -

Verhoeven M, Bystrova SN, Winnubst L, Qureshi H, Gruijl, de TD, Scheper RJ et al. Applying ceramic nanoporous microneedle arrays as a transport interface in egg plants and an ex-vivo human skin model. Microelectronic Engineering. 2012;98:659-662. Available from, DOI: 10.1016/j.mee.2012.07.022