Applications of 31P NMR in the study of microbial metabolism

K. Nicolaij

Research output: Contribution to journalArticleAcademicpeer-review


31P NMR has been shown to be a useful method for the study of microbial metabolism in vivo. This is mainly due to the fact that a variety of important metabolites, such as ATP, are phosphorylated, and that the31P nucleus has a 100% natural abundance. The concentrations of phosphorylated metabolites and time-dependent changes in metabolism can be monitored non-invasively by31P NMR. Furthermore, the resonance positions of some of these molecules are sensitive to the local ionic environment and, thus, can be used to estimate cellular pH. Saturation-transfer31P NMR allows the measurement of fluxes through certain enzymatic reactions under steady-state conditions. Since such parameters are measured in the intact cell.31P NMR has led to new insights into the regulation of cellular processes.
Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalTrends in Food Science and Technology
Publication statusPublished - 1992


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